Application of Coherent Anti-stokes Raman Scattering (CARS) to imaging mammalian cells: A means for gaining molecular selectivity in multiphoton imaging

Gary Holtom; Brian Thrall; Thomas Weber; Leyun Zhu; Derek Hopkins; Christopher Parkinson; Steven Colson; John Price; Beek Yoke Chin; Augustine M K Choi; Terence Risby

doi:10.1117/12.424571

Application of Coherent Anti-stokes Raman Scattering (CARS) to imaging mammalian cells: A means for gaining molecular selectivity in multiphoton imaging

Gary Holtom, Brian Thrall, Thomas Weber, Leyun Zhu, Derek Hopkins, Christopher Parkinson, Steven Colson, John Price, Beek Yoke Chin, Augustine M K Choi , Terence Risby

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Coherent anti-Stokes Raman scattering (CARS) was employed to provide molecular selectivity in the imaging of mammalian cells. The method involved multiphoton fluorescence achieved by using ultrafast laser pulses. The addition of a second laser beam increased the detected Raman signal by many orders of magnitude besides removing fluorescence artifacts. An optical parametric amplifier and confocal microscopy was also utilized for the imaging process.

Original language	English (US)
Pages (from-to)	319-328
Number of pages	10
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4262
DOIs	https://doi.org/10.1117/12.424571
State	Published - 2001

Keywords

CARS
Confocal microscopy
Multiphoton fluorescence
Raman imaging

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.424571

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Holtom, G., Thrall, B., Weber, T., Zhu, L., Hopkins, D., Parkinson, C., Colson, S., Price, J., Chin, B. Y., Choi , A. M. K., & Risby, T. (2001). Application of Coherent Anti-stokes Raman Scattering (CARS) to imaging mammalian cells: A means for gaining molecular selectivity in multiphoton imaging. Proceedings of SPIE - The International Society for Optical Engineering, 4262, 319-328. https://doi.org/10.1117/12.424571

Application of Coherent Anti-stokes Raman Scattering (CARS) to imaging mammalian cells: A means for gaining molecular selectivity in multiphoton imaging. / Holtom, Gary; Thrall, Brian; Weber, Thomas et al.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 4262, 2001, p. 319-328.

Research output: Contribution to journal › Article › peer-review

Holtom, G, Thrall, B, Weber, T, Zhu, L, Hopkins, D, Parkinson, C, Colson, S, Price, J, Chin, BY, Choi , AMK & Risby, T 2001, 'Application of Coherent Anti-stokes Raman Scattering (CARS) to imaging mammalian cells: A means for gaining molecular selectivity in multiphoton imaging', Proceedings of SPIE - The International Society for Optical Engineering, vol. 4262, pp. 319-328. https://doi.org/10.1117/12.424571

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AU - Price, John

AU - Chin, Beek Yoke

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AU - Risby, Terence

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Application of Coherent Anti-stokes Raman Scattering (CARS) to imaging mammalian cells: A means for gaining molecular selectivity in multiphoton imaging

Abstract

Keywords

ASJC Scopus subject areas

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