Apparent continuity between the messenger transport organizer and late RNA localization pathways during oogenesis in Xenopus

Malgorzata Kloc, Laurence D. Etkin

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

The localization of RNAs at the vegetal cortex in Xenopus oocytes is a complex process, involving at least two different pathways. The early, or messenger transport organizer (METRO), pathway, localizes RNAs such as Xlsirts, Xcat2 and Xwnt11 during stages 1 and 2 of oogenesis, while the late pathway localizes RNAs such as Vg1 during stages 2-4. We demonstrate that the onset of Vg1 localization is characterized by its microtubule-independent binding to a subdomain of the endoplasmic reticulum (ER). The formation of this unique ER structure is intimately associated with the movement of the mitochondrial cloud toward the vegetal cortex. In addition, we demonstrate that the mitochondrial cloud contains a γ-tubulin-positive structure that may function as a microtubule organizing center for establishing microtubule tracks for Vg1 localization. These data, support, although they do not prove, a model in which the development of the late pathway machinery relies upon the prior functioning of the early pathway.

Original languageEnglish (US)
Pages (from-to)95-106
Number of pages12
JournalMechanisms of Development
Volume73
Issue number1
DOIs
StatePublished - Apr 1 1998

Keywords

  • Axial patterning
  • Development
  • Oocyte polarity
  • RNA localization
  • Vegetal cortex
  • Xenopus laevis

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

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