ApoE and the role of very low density lipoproteins in adipose tissue inflammation

Jiali Wang, Xiaoyuan Dai Perrard, Jerry L. Perrard, Aparna Mukherjee, Corina Rosales, Yuguo Chen, C. Wayne Smith, Henry J. Pownall, Christie M. Ballantyne, Huaizhu Wu

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Objective: To identify the role of triglyceride-rich lipoproteins (TGRLs) and apoE, a major apolipoprotein in TGRLs, in adipose tissue inflammation with high-fat diet (HFD)-induced obesity. Methods: Male apoE-/- and C57BL/6J wild-type (WT) mice fed HFD for 12 weeks were assessed for metabolic and inflammatory parameters. ApoE-/- and WT mice were orally gavaged with [3H]palmitic acid to examine the role of apoE in fat delivery to adipose tissue. VLDL from obese apoE-/- mice were intravenously injected into lean WT or apoE-/- mice to test potential contribution of TGRLs-derived fat delivery to inflammation in adipose tissue and the role of apoE. Results: ApoE-/- mice gained less body weight, and had less fat mass and lower triglyceride levels in skeletal muscle than WT. ApoE-/- mice on HFD had better insulin sensitivity than WT even when comparing body weight-matched mice. Compared to WT mice, apoE-/- mice on HFD had lower levels of inflammatory cytokines/chemokines and CD11c in adipose tissue, and lower levels of inflammatory markers in skeletal muscle. At 6 h after oral gavage with [3H]palmitic acid, incorporation of [3H]palmitic acid into adipose tissue and skeletal muscle was lower in apoE-/- mice. After repeated daily injection for 3 days, VLDL from obese apoE-/- mice induced inflammation in adipose tissue of recipient WT but not apoE-/- mice. Conclusion: In HFD-induced obesity, apoE plays an important role in inflammation in adipose tissue and skeletal muscle, likely by mediating TGRL-derived fat delivery to these tissues.

Original languageEnglish (US)
Pages (from-to)342-349
Number of pages8
Issue number2
StatePublished - Aug 2012


  • Adipose tissue
  • Apolipoprotein E
  • Inflammation
  • Insulin resistance
  • Lipoproteins
  • Obesity

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine


Dive into the research topics of 'ApoE and the role of very low density lipoproteins in adipose tissue inflammation'. Together they form a unique fingerprint.

Cite this