Antioxidative function and biodistribution of [Gd@C82(OH) 22]n nanoparticles in tumor-bearing mice

Jiangxue Wang, Chunying Chen, Bai Li, Hongwei Yu, Yuliang Zhao, Jin Sun, Yufeng Li, Gengmei Xing, Hui Yuan, Jun Tang, Zhen Chen, Huan Meng, Yuxi Gao, Chang Ye, Zhifang Chai, Chuanfeng Zhu, Baocheng Ma, Xiaohong Fang, Lijun Wan

Research output: Contribution to journalArticle

147 Scopus citations

Abstract

Oxidative stress is considered to be one of the important mechanisms involved in carcinogenesis. In our previous study, gadolinium endohedral metallofullerenol ([Gd@C82(OH)22]n nanoparticles) have shown high inhibitory activity on hepatoma cell (H22) growth in mice. To explore the antioxidative functions of nanoparticles, we investigated the biodistribution of [Gd@C82(OH)22] n nanoparticles, the changes of blood coagulation profiles, the metabolism of reactive oxygen species (ROS) in the tumor-bearing mice as well as the possible relationships between nanoparticles treatment and ROS production in this paper. The activities of hepatic superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST) and catalase (CAT) as well as the levels of reduced glutathione (GSH), protein-bound thiols and malondialdehyde (MDA) were compared between the tumor-bearing mice and normal mice. Transplanted tumors were grown in mice by subcutaneous injection of murine hepatoma cells in the mice. The comparison of the above parameters between nanoparticles and cyclophosphamide (CTX) therapy were also investigated. [Gd@C82(OH)22]n administration can efficiently restore the damaged liver and kidney of the tumor-bearing mice. All the activities of enzymes and other parameters related to oxidative stress were reduced after [Gd@C82(OH)22]n treatment and tended closely to the normal levels. The results suggest that [Gd@C 82(OH)22]n nanoparticle treatment could regulate ROS production in vivo.

Original languageEnglish (US)
Pages (from-to)872-881
Number of pages10
JournalBiochemical pharmacology
Volume71
Issue number6
DOIs
StatePublished - Mar 14 2006

Keywords

  • [Gd@C(OH)] nanoparticles
  • Antioxidant
  • Biodistribution
  • In vivo
  • Oxidative stress

ASJC Scopus subject areas

  • Pharmacology

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