Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis

Rodnei Dennis Rossoni; Patrícia Pimentel de Barros; Janaina Araújo de Alvarenga; Felipe de Camargo Ribeiro; Marisol dos Santos Velloso; Beth Burgwyn Fuchs; Eleftherios Mylonakis; Antonio Olavo Cardoso Jorge; Juliana Campos Junqueira

doi:10.1080/08927014.2018.1425402

Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis

Rodnei Dennis Rossoni, Patrícia Pimentel de Barros, Janaina Araújo de Alvarenga, Felipe de Camargo Ribeiro, Marisol dos Santos Velloso, Beth Burgwyn Fuchs, Eleftherios Mylonakis, Antonio Olavo Cardoso Jorge, Juliana Campos Junqueira

Houston Methodist

Research output: Contribution to journal › Article › peer-review

93 Scopus citations

Abstract

This study isolated Lactobacillus strains from caries-free subjects and evaluated the inhibitory effects directly on three strains of C. albicans, two clinical strains and one reference strain. Thirty Lactobacillus strains were isolated and evaluated for antimicrobial activity against in vitro C. albicans biofilms. L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 isolates exhibited the most significant inhibitory activity against C. albicans. Co-incubation between these microorganisms resulted in deterrence of biofilm development and retardation of hyphal formation. The hindrance of biofilm development was characterized by the downregulated expression of C. albicans biofilm-specific genes (ALS3, HWP1, EFG1 and CPH1). L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 demonstrated the ability to exert antifungal activity through the inhibition of C. albicans biofilms.

Original language	English (US)
Pages (from-to)	212-225
Number of pages	14
Journal	Biofouling
Volume	34
Issue number	2
DOIs	https://doi.org/10.1080/08927014.2018.1425402
State	Published - Feb 7 2018

Keywords

Biofilm
Candida albicans
Lactobacillus
mycology
pathogenesis

ASJC Scopus subject areas

Aquatic Science
Applied Microbiology and Biotechnology
Water Science and Technology

Divisions

Infectious Disease

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10.1080/08927014.2018.1425402

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Rossoni, R. D., de Barros, P. P., de Alvarenga, J. A., Ribeiro, F. D. C., Velloso, M. D. S., Fuchs, B. B., Mylonakis, E., Jorge, A. O. C., & Junqueira, J. C. (2018). Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis. Biofouling, 34(2), 212-225. https://doi.org/10.1080/08927014.2018.1425402

Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis. / Rossoni, Rodnei Dennis; de Barros, Patrícia Pimentel; de Alvarenga, Janaina Araújo et al.
In: Biofouling, Vol. 34, No. 2, 07.02.2018, p. 212-225.

Research output: Contribution to journal › Article › peer-review

Rossoni, RD, de Barros, PP, de Alvarenga, JA, Ribeiro, FDC, Velloso, MDS, Fuchs, BB, Mylonakis, E, Jorge, AOC & Junqueira, JC 2018, 'Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis', Biofouling, vol. 34, no. 2, pp. 212-225. https://doi.org/10.1080/08927014.2018.1425402

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abstract = "This study isolated Lactobacillus strains from caries-free subjects and evaluated the inhibitory effects directly on three strains of C. albicans, two clinical strains and one reference strain. Thirty Lactobacillus strains were isolated and evaluated for antimicrobial activity against in vitro C. albicans biofilms. L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 isolates exhibited the most significant inhibitory activity against C. albicans. Co-incubation between these microorganisms resulted in deterrence of biofilm development and retardation of hyphal formation. The hindrance of biofilm development was characterized by the downregulated expression of C. albicans biofilm-specific genes (ALS3, HWP1, EFG1 and CPH1). L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 demonstrated the ability to exert antifungal activity through the inhibition of C. albicans biofilms.",

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AB - This study isolated Lactobacillus strains from caries-free subjects and evaluated the inhibitory effects directly on three strains of C. albicans, two clinical strains and one reference strain. Thirty Lactobacillus strains were isolated and evaluated for antimicrobial activity against in vitro C. albicans biofilms. L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 isolates exhibited the most significant inhibitory activity against C. albicans. Co-incubation between these microorganisms resulted in deterrence of biofilm development and retardation of hyphal formation. The hindrance of biofilm development was characterized by the downregulated expression of C. albicans biofilm-specific genes (ALS3, HWP1, EFG1 and CPH1). L. paracasei 28.4, L. rhamnosus 5.2 and L. fermentum 20.4 demonstrated the ability to exert antifungal activity through the inhibition of C. albicans biofilms.

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Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral candidiasis

Abstract

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