Antibody-free detection of Mycobacterium tuberculosis antigen using customized nanotraps

Research output: Contribution to journalArticle

Hung Jen Wu, Yaojun Li, Jia Fan, Zaian Deng, Zhao Hu, Xuewu Liu, Edward A. Graviss, Mauro Ferrari, Xin Ma, Ye Hu

Rapid screening and diagnosis of tuberculosis disease (TB) is still challenging and critically needed for global TB control efforts. In this study, we present a rapid and streamlined technology, using precisely engineered silica nanopore thin films, which are optimized for pore size, structure, capillary force, and film thickness, to isolate Mycobacterium tuberculosis (MTB) antigens in laboratory and clinical samples for rapid TB screening. This technology, referred to here as on-chip fractionation, is integrated with high-throughput matrix-assisted laser desorption/ionization time-of flight mass spectrometry to screen and identify fragments of the MTB antigen, CFP-10, from complex biological samples, without use of immunoaffinity agents. With the use of this comprehensive approach, we were able to clearly distinguish a clinical isolate of MTB from a nonTB species of the genus Mycobacterium avium grown in liquid culture media. This assay can reach a detection limit of 10 fmol and an isolation rate of 90% for the antigen CFP-10. Our strategy has significant potential to fill the conceptual and technical gaps in rapid diagnosis of active TB disease.

Original languageEnglish
Pages (from-to)1988-1996
Number of pages9
JournalAnalytical Chemistry
Volume86
Issue number4
DOIs
StatePublished - Feb 18 2014

PMID: 24446580

PMCID: PMC3983014

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Antibody-free detection of Mycobacterium tuberculosis antigen using customized nanotraps. / Wu, Hung Jen; Li, Yaojun; Fan, Jia; Deng, Zaian; Hu, Zhao; Liu, Xuewu; Graviss, Edward A.; Ferrari, Mauro; Ma, Xin; Hu, Ye.

In: Analytical Chemistry, Vol. 86, No. 4, 18.02.2014, p. 1988-1996.

Research output: Contribution to journalArticle

Harvard

Wu, HJ, Li, Y, Fan, J, Deng, Z, Hu, Z, Liu, X, Graviss, EA, Ferrari, M, Ma, X & Hu, Y 2014, 'Antibody-free detection of Mycobacterium tuberculosis antigen using customized nanotraps' Analytical Chemistry, vol. 86, no. 4, pp. 1988-1996. https://doi.org/10.1021/ac4027669

APA

Wu, H. J., Li, Y., Fan, J., Deng, Z., Hu, Z., Liu, X., ... Hu, Y. (2014). Antibody-free detection of Mycobacterium tuberculosis antigen using customized nanotraps. Analytical Chemistry, 86(4), 1988-1996. https://doi.org/10.1021/ac4027669

Vancouver

Wu HJ, Li Y, Fan J, Deng Z, Hu Z, Liu X et al. Antibody-free detection of Mycobacterium tuberculosis antigen using customized nanotraps. Analytical Chemistry. 2014 Feb 18;86(4):1988-1996. https://doi.org/10.1021/ac4027669

Author

Wu, Hung Jen ; Li, Yaojun ; Fan, Jia ; Deng, Zaian ; Hu, Zhao ; Liu, Xuewu ; Graviss, Edward A. ; Ferrari, Mauro ; Ma, Xin ; Hu, Ye. / Antibody-free detection of Mycobacterium tuberculosis antigen using customized nanotraps. In: Analytical Chemistry. 2014 ; Vol. 86, No. 4. pp. 1988-1996.

BibTeX

@article{5dee9f68979749389b84cd70a48e79f5,
title = "Antibody-free detection of Mycobacterium tuberculosis antigen using customized nanotraps",
abstract = "Rapid screening and diagnosis of tuberculosis disease (TB) is still challenging and critically needed for global TB control efforts. In this study, we present a rapid and streamlined technology, using precisely engineered silica nanopore thin films, which are optimized for pore size, structure, capillary force, and film thickness, to isolate Mycobacterium tuberculosis (MTB) antigens in laboratory and clinical samples for rapid TB screening. This technology, referred to here as on-chip fractionation, is integrated with high-throughput matrix-assisted laser desorption/ionization time-of flight mass spectrometry to screen and identify fragments of the MTB antigen, CFP-10, from complex biological samples, without use of immunoaffinity agents. With the use of this comprehensive approach, we were able to clearly distinguish a clinical isolate of MTB from a nonTB species of the genus Mycobacterium avium grown in liquid culture media. This assay can reach a detection limit of 10 fmol and an isolation rate of 90{\%} for the antigen CFP-10. Our strategy has significant potential to fill the conceptual and technical gaps in rapid diagnosis of active TB disease.",
author = "Wu, {Hung Jen} and Yaojun Li and Jia Fan and Zaian Deng and Zhao Hu and Xuewu Liu and Graviss, {Edward A.} and Mauro Ferrari and Xin Ma and Ye Hu",
year = "2014",
month = "2",
day = "18",
doi = "10.1021/ac4027669",
language = "English",
volume = "86",
pages = "1988--1996",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "4",

}

RIS

TY - JOUR

T1 - Antibody-free detection of Mycobacterium tuberculosis antigen using customized nanotraps

AU - Wu, Hung Jen

AU - Li, Yaojun

AU - Fan, Jia

AU - Deng, Zaian

AU - Hu, Zhao

AU - Liu, Xuewu

AU - Graviss, Edward A.

AU - Ferrari, Mauro

AU - Ma, Xin

AU - Hu, Ye

PY - 2014/2/18

Y1 - 2014/2/18

N2 - Rapid screening and diagnosis of tuberculosis disease (TB) is still challenging and critically needed for global TB control efforts. In this study, we present a rapid and streamlined technology, using precisely engineered silica nanopore thin films, which are optimized for pore size, structure, capillary force, and film thickness, to isolate Mycobacterium tuberculosis (MTB) antigens in laboratory and clinical samples for rapid TB screening. This technology, referred to here as on-chip fractionation, is integrated with high-throughput matrix-assisted laser desorption/ionization time-of flight mass spectrometry to screen and identify fragments of the MTB antigen, CFP-10, from complex biological samples, without use of immunoaffinity agents. With the use of this comprehensive approach, we were able to clearly distinguish a clinical isolate of MTB from a nonTB species of the genus Mycobacterium avium grown in liquid culture media. This assay can reach a detection limit of 10 fmol and an isolation rate of 90% for the antigen CFP-10. Our strategy has significant potential to fill the conceptual and technical gaps in rapid diagnosis of active TB disease.

AB - Rapid screening and diagnosis of tuberculosis disease (TB) is still challenging and critically needed for global TB control efforts. In this study, we present a rapid and streamlined technology, using precisely engineered silica nanopore thin films, which are optimized for pore size, structure, capillary force, and film thickness, to isolate Mycobacterium tuberculosis (MTB) antigens in laboratory and clinical samples for rapid TB screening. This technology, referred to here as on-chip fractionation, is integrated with high-throughput matrix-assisted laser desorption/ionization time-of flight mass spectrometry to screen and identify fragments of the MTB antigen, CFP-10, from complex biological samples, without use of immunoaffinity agents. With the use of this comprehensive approach, we were able to clearly distinguish a clinical isolate of MTB from a nonTB species of the genus Mycobacterium avium grown in liquid culture media. This assay can reach a detection limit of 10 fmol and an isolation rate of 90% for the antigen CFP-10. Our strategy has significant potential to fill the conceptual and technical gaps in rapid diagnosis of active TB disease.

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UR - http://www.scopus.com/inward/citedby.url?scp=84894250876&partnerID=8YFLogxK

U2 - 10.1021/ac4027669

DO - 10.1021/ac4027669

M3 - Article

VL - 86

SP - 1988

EP - 1996

JO - Analytical Chemistry

T2 - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 4

ER -

ID: 2831805