Antibody response to fibronectin-binding adhesin FnbpA in patients with Staphylococcus aureus infections

Fabrizia Casolini, Livia Visai, Danny Joh, Pier Giulio Conaldi, Antonio Toniolo, Magnus Höök, Pietro Speziale

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

We have analyzed antibody reactivity to a fibronectin-binding microbial surface component that recognizes adhesive matrix molecules (MSCRAMM) in blood plasma collected from patients with staphylococcal infections. All patients had elevated levels of anti-MSCRAMM antibodies compared to those of young children who, presumably, had not been exposed to staphylococcal infections. The anti-MSCRAMM antibodies preferentially reacted with the ligand-binding repeat domain of the adhesin. However, these antibodies did not inhibit fibronectin binding. Essentially, all patients had antibodies which specifically recognized the fibronectin-MSCRAMM complex but not the isolated components. Epitopes recognized by these anti-ligand-induced binding sites antibodies were found in each repeat unit of the MSCRAMM. These results demonstrate that staphylococci have bound fibronectin some time during infection and that each repeat unit in the MSCRAMM can engage in ligand binding. Furthermore, our previously proposed model, suggesting that an unordered structure in the MSCRAMM undergoes a conformational change upon ligand binding (K. House-Pompeo, Y. Xu, D. Joh, P. Speziale, and M. Hook, J. Biol. Chem. 271:1379-1384, 1996), is presumably operational in patients during infections.

Original languageEnglish (US)
Pages (from-to)5433-5442
Number of pages10
JournalInfection and Immunity
Volume66
Issue number11
DOIs
StatePublished - 1998

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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