Antibody-mediated inhibition of Nogo-A signaling promotes neurite growth in PC-12 cells

Iman K. Yazdi, Nima Taghipour, Sarah Hmaidan, Roberto Palomba, Shilpa Scaria, Alvaro Munoz, Timothy B. Boone, Ennio Tasciotti

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The use of a monoclonal antibody to block the neurite outgrowth inhibitor Nogo-A has been of great interest for promoting axonal recovery as a treatment for spinal cord injury. While several cellular and non-cellular assays have been developed to quantify the bioactive effects of Nogo-A signaling, demand still exists for the development of a reliable approach to characterize the effectiveness of the anti-Nogo-A antibody. In this study, we developed and validated a novel cell-based approach to facilitate the biological quantification of a Nogo-A antibody using PC-12 cells as an in vitro neuronal cell model. Changes in the mRNA levels of the neuronal differentiation markers, growth-associated protein 43 and neurofilament light-polypeptide, suggest that activation of the Nogo-A pathway suppresses axonal growth and dendrite formation in the tested cell line. We found that application of anti-Nogo-A monoclonal antibody can significantly enhance the neuronal maturity of PC-12 cells by blocking the Nogo-A inhibitory effects, providing enhanced effects on neural maturity at the molecular level. No adverse effects were observed on cell viability.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalJournal of Tissue Engineering
Volume7
DOIs
StatePublished - Feb 16 2016

Keywords

  • Nogo-A
  • Bioactivity
  • Neuroregeneration
  • PC-12 cells

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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