Antibody-mediated delivery of sirnas for anti-hiv therapy

Sang Soo Kim, Sandesh Subramanya, Dan Peer, Motomu Shimaoka, Premlata Shankar

Research output: Chapter in Book/Report/Conference proceedingChapter

19 Scopus citations

Abstract

RNA interference (RNAi) is a potent and specific gene silencing mechanism that utilizes small -double-stranded RNA intermediates (small interfering RNAs or siRNAs) to target homologous mRNA sequences for degradation. The therapeutic potential of RNAi for HIV infection has been demonstrated in many studies. However, successful clinical application of RNAi is contingent on developing practical strategies to deliver siRNA to the desired target cells and tissues. Recently, there has been significant progress towards developing reagents that selectively deliver exogenous siRNA to immune cells that are targeted by HIV or involved in viral pathogenesis, such as T cells, macrophages, and dendritic cells. Here, we describe details of two antibody-based strategies for systemic delivery of siRNA either specifically to T cells via the CD7 receptor or to multiple immune cell types via LFA-1, present on all leukocytes.

Original languageEnglish (US)
Title of host publicationAntiviral RNAi
Subtitle of host publicationConcepts, Methods, and Applications
EditorsRonald Rij
Pages339-353
Number of pages15
DOIs
StatePublished - Dec 1 2011

Publication series

NameMethods in Molecular Biology
Volume721
ISSN (Print)1064-3745

Keywords

  • Cationic peptide
  • HIV
  • I-tsNPs (integrin-targeted stabilized nanoparticles)
  • Integrin
  • LFA-1 (lymphocyte function-associated antigen-1)
  • Liposome
  • RNA interference
  • scFvCD7
  • Small interfering RNA
  • Targeted delivery

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Fingerprint Dive into the research topics of 'Antibody-mediated delivery of sirnas for anti-hiv therapy'. Together they form a unique fingerprint.

Cite this