Antibodies encoded by FCRL4-bearing memory B cells preferentially recognize commensal microbial antigens

Yanling Liu; Jonathan R. McDaniel; Srijit Khan; Paolo Campisi; Evan J. Propst; Theresa Holler; Eyal Grunebaum; George Georgiou; Gregory C. Ippolito; Götz R.A. Ehrhardt

doi:10.4049/jimmunol.1701549

Antibodies encoded by FCRL4-bearing memory B cells preferentially recognize commensal microbial antigens

Yanling Liu, Jonathan R. McDaniel, Srijit Khan, Paolo Campisi, Evan J. Propst, Theresa Holler, Eyal Grunebaum, George Georgiou, Gregory C. Ippolito, Götz R.A. Ehrhardt

Academic Institute

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

FCRL4, a low-affinity IgA Ab receptor with strong immunoregulatory potential, is an identifying feature of a tissue-based population of memory B cells (Bmem). We used two independent approaches to perform a comparative analysis of the Ag receptor repertoires of FCRL4 ⁺ and FCRL4 ^- Bmem in human tonsils. We determined that FCRL4 ⁺ Bmem displayed lower levels of somatic mutations in their Ag receptors compared with FCRL4 ^- Bmem but had similar frequencies of variable gene family usage. Importantly, Abs with reactivity to commensal microbiota were enriched in FCRL4+ cells, a phenotype not due to polyreactive binding characteristics. Our study links expression of the immunoregulatory FCRL4 molecule with increased recognition of commensal microbial Ags.

Original language	English (US)
Pages (from-to)	3962-3969
Number of pages	8
Journal	Journal of Immunology
Volume	200
Issue number	12
DOIs	https://doi.org/10.4049/jimmunol.1701549
State	Published - Jun 15 2018

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "Antibodies encoded by FCRL4-bearing memory B cells preferentially recognize commensal microbial antigens",

abstract = " FCRL4, a low-affinity IgA Ab receptor with strong immunoregulatory potential, is an identifying feature of a tissue-based population of memory B cells (Bmem). We used two independent approaches to perform a comparative analysis of the Ag receptor repertoires of FCRL4 + and FCRL4 - Bmem in human tonsils. We determined that FCRL4 + Bmem displayed lower levels of somatic mutations in their Ag receptors compared with FCRL4 - Bmem but had similar frequencies of variable gene family usage. Importantly, Abs with reactivity to commensal microbiota were enriched in FCRL4+ cells, a phenotype not due to polyreactive binding characteristics. Our study links expression of the immunoregulatory FCRL4 molecule with increased recognition of commensal microbial Ags.",

author = "Yanling Liu and McDaniel, {Jonathan R.} and Srijit Khan and Paolo Campisi and Propst, {Evan J.} and Theresa Holler and Eyal Grunebaum and George Georgiou and Ippolito, {Gregory C.} and Ehrhardt, {G{\"o}tz R.A.}",

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year = "2018",

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doi = "10.4049/jimmunol.1701549",

language = "English (US)",

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T1 - Antibodies encoded by FCRL4-bearing memory B cells preferentially recognize commensal microbial antigens

AU - Liu, Yanling

AU - McDaniel, Jonathan R.

AU - Khan, Srijit

AU - Campisi, Paolo

AU - Propst, Evan J.

AU - Holler, Theresa

AU - Grunebaum, Eyal

AU - Georgiou, George

AU - Ippolito, Gregory C.

AU - Ehrhardt, Götz R.A.

PY - 2018/6/15

Y1 - 2018/6/15

N2 - FCRL4, a low-affinity IgA Ab receptor with strong immunoregulatory potential, is an identifying feature of a tissue-based population of memory B cells (Bmem). We used two independent approaches to perform a comparative analysis of the Ag receptor repertoires of FCRL4 + and FCRL4 - Bmem in human tonsils. We determined that FCRL4 + Bmem displayed lower levels of somatic mutations in their Ag receptors compared with FCRL4 - Bmem but had similar frequencies of variable gene family usage. Importantly, Abs with reactivity to commensal microbiota were enriched in FCRL4+ cells, a phenotype not due to polyreactive binding characteristics. Our study links expression of the immunoregulatory FCRL4 molecule with increased recognition of commensal microbial Ags.

AB - FCRL4, a low-affinity IgA Ab receptor with strong immunoregulatory potential, is an identifying feature of a tissue-based population of memory B cells (Bmem). We used two independent approaches to perform a comparative analysis of the Ag receptor repertoires of FCRL4 + and FCRL4 - Bmem in human tonsils. We determined that FCRL4 + Bmem displayed lower levels of somatic mutations in their Ag receptors compared with FCRL4 - Bmem but had similar frequencies of variable gene family usage. Importantly, Abs with reactivity to commensal microbiota were enriched in FCRL4+ cells, a phenotype not due to polyreactive binding characteristics. Our study links expression of the immunoregulatory FCRL4 molecule with increased recognition of commensal microbial Ags.

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Antibodies encoded by FCRL4-bearing memory B cells preferentially recognize commensal microbial antigens

Abstract

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