Anti-TNFα therapy improves survival and ameliorates the pathophysiologic sequelae in acute pancreatitis in the rat

Christopher B. Hughes, Hani P. Grewal, Lillian Gaber, Malak Kotb, Abou Bakr Mohey El-Din, Linda Mann, A. Osama Gaber

Research output: Contribution to journalArticle

128 Scopus citations

Abstract

BACKGROUND: Elevated levels of tumor necrosis factor-alpha (TNFα) have been measured in a lethal model of acute pancreatitis (AP) and may contribute to the pathophysiologic sequelae of the disease. METHODS: To determine the significance of anti-TNFα therapy on survival and disease manifestations in a clinically relevant model of AP, a rat model was developed using a retrograde pancreatic ductal infusion of bile. Animals were randomized to no treatment (n = 30) or treatment with anti-TNFα antibody 15 minutes prior to induction of AP (n = 30). Five treated and 5 untreated rats were killed at various time periods up to 72 hours to provide temporal characterization of TNFα activity in AP. RESULTS: A burst of TNFα activity in the serum of untreated pancreatitis animals between 1 and 3 hours after induction of the disease is prevented by pretreatment with anti-TNFα antibody. CONCLUSIONS: These findings provide a plausible mechanism for the improvement in biochemical and histologic parameters as well as in overall survival in an experimental model of acute pancreatitis in the rat.

Original languageEnglish (US)
Pages (from-to)274-280
Number of pages7
JournalAmerican Journal of Surgery
Volume171
Issue number2
DOIs
StatePublished - Jan 1 1996

ASJC Scopus subject areas

  • Surgery

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