Anti-idiotypic T-cell activation in multiple myeloma induced by M- component fragments presented by dendritic cells

Sunil Dabadghao, Susanne Bergenbrant, Doina Anton, Wen He, Göran Holm, Qing Yi

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

The monoclonal immunoglobulin (Ig) (M-component) secreted by the tumour plasma cells in multiple myeloma (MM) has specific antigenic determinants (idiotypes; id) that can serve as tumour-specific antigens. The intact Ig molecule is a weak antigen, and small fragments of id protein might be more immunogenic for the induction of id-specific immunity. Dendritic cells (DC) have attracted attention as the most efficient antigen-presenting cells and promising adjuvants for immunotherapy in tumours. In this study the in vitro T-cell response against F(ab')2 and Fab fragments, heavy and light: chains of the M-component was examined in five patients with MM clinical stage I. All fragments were able to stimulate T cells but F(ab')2 or Fab fragments and heavy chains induced a stronger response than light chains. DC induced a significantly stronger id-specific immune response than monocytes. Moreover, with DC as antigenpresenting cells, a predominant interferon (IFN)-χ, (type- 1 T-cell) response was seen in all patients. Both IFN-χ, and interleukin (IL)-4 (type-1 and type-2 T-cell) responses were noted when monocytes were used. Our study suggests that DC pulsed with idiotypic fragments such as F(ab')2 fragment and heavy chain can be used for the induction of type-1 antiidiotypic T-cell response for immunotherapy in MM.

Original languageEnglish (US)
Pages (from-to)647-654
Number of pages8
JournalBritish Journal of Haematology
Volume100
Issue number4
DOIs
StatePublished - Mar 31 1998

Keywords

  • Antiidiotypic T subsets
  • Dendritic cells
  • Idiotype
  • Multiple myeloma

ASJC Scopus subject areas

  • Hematology

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