Anti-Ace monoclonal antibody reduces Enterococcus faecalis aortic valve infection in a rat infective endocarditis model

Kavindra V. Singh, Kenneth L. Pinkston, Peng Gao, Barrett R. Harvey, Barbara E. Murray

Research output: Contribution to journalArticlepeer-review

Abstract

Ace (Adhesin to collagen from Enterococcus faecalis) is a cell-wall anchored protein that is expressed conditionally and is important for virulence in a rat infective endocarditis (IE) model. Previously, we showed that rats immunized with the collagen binding domain of Ace (domain A), or administered anti-Ace domain A polyclonal antibody, were less susceptible to E. faecalis endocarditis than sham-immunized controls. In this work, we demonstrated that a sub nanomolar monoclonal antibody (mAb), anti-Ace mAb 70, significantly diminished E. faecalis binding to ECM collagen IV in in vitro adherence assays and that, in the endocarditis model, anti-Ace mAb 70 pre-treatment significantly reduced E. faecalis infection of aortic valves. The effectiveness of anti-Ace mAb against IE in the rat model suggests it might serve as a beneficial agent for passive protection against E. faecalis infections.

Original languageEnglish (US)
Article numberfty084
JournalPathogens and Disease
Volume76
Issue number8
DOIs
StatePublished - Nov 1 2018

Keywords

  • ace collagen adhesion
  • Enterococcus faecalis
  • immunoprophylaxis
  • infective endocarditis
  • pathogenesis
  • protective vaccine

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

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