Antagonism of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) - induced cyp1A1 gene expression by 6-methyl-1,3,8-trichlorodibenzofuran (MCDF): Mechanistic studies

Mark Merchant, Valerie Morrison, Stephen Safe

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Treatment of rat hepatoma H-4-II E cells with 10-9 M 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) resulted in maximal induction of cytochrome P4501A1-dependent ethoxyresorufin O-deethylase (EROD) activity. In contrast, 10-6 M 6-methyl-1,3,8-trichlorodibenzofuran (MCDF) was relatively inactive as an inducer. Cotreatment with TCDD (10-9 M) and MCDF (10-8, 10-7, 10-6 M) resulted in a concentration-dependent antagonism of EROD induction. In addition, MCDF caused a concentration-dependent decrease in TCDD-induced CYP1A1 mRNA levels. In a parallel study, MCDF reduced the accumulation of transcriptionally active nuclear [3H]TCDD:Ah receptor complexes. Receptor competition studies with freshly prepared Sprague-Dawley rat cytosol revealed that MCDF competes with [3H]TCDD for Ah receptor binding sites (EC50 ≈ 140 nM). These results suggest that MCDF antagonizes TCDD-mediated induction of CYP1A1 gene expression by competition for the Ah receptor and by decreasing the accumulation of transcriptionally active nuclear Ah receptor complexes.

Original languageEnglish (US)
Pages (from-to)967-973
Number of pages7
JournalChemosphere
Volume25
Issue number7-10
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

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