Androgen metabolism in rat anterior pituitary cells in culture

A. Belanger; Lena Lundell; P. Eneroth; J. A. Gustafsson

doi:10.1016/0303-7207(80)90020-9

Androgen metabolism in rat anterior pituitary cells in culture

A. Belanger, Lena Lundell, P. Eneroth, J. A. Gustafsson

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Abstract

The metabolism in vitro of 4 androgens, namely testosterone, androstenedione, 5α-dihydrotestosterone and 3α-androstanediol has been studied in male and female rat anterior pituitary cells in primary culture. When testosterone was used as precursor, androstenedione, 5α-dihydrotestosterone and 3α-androstanediol were the main metabolites whereas androstenedione was mainly converted into testosterone, 5α-androstanedione, 5α-dihydrotestosterone and androsterone. Studies on the metabolism of 5α-dihydrotestosterone and 3α-androstanediol showed that these compounds were easily interconverted and were also significantly metabolized to 5α-androstanedione and androsterone. No aromatized compounds could be detected suggesting that androgen action in the pituitary cell occurs directly via the androgen receptor rather than through prior conversion into estrogens.

Original language	English (US)
Pages (from-to)	175-181
Number of pages	7
Journal	Molecular and cellular endocrinology
Volume	19
Issue number	2
DOIs	https://doi.org/10.1016/0303-7207(80)90020-9
State	Published - 1980

Keywords

17β-dehydrogenase
3α-Adiol, (3α,17β-dihydroxy-5α-androstane)
3α-Adiol, (3α,17β-dihydroxy-5α-androstane), androst-4-ene-3,17-dione)
3α-androstanediol
3α-androstanediol
3α-dehydrogenase
3β-Adiol (3β,17β-dihydroxy-5αx-androstane)
3β-androstanediol
3β-dehydrogenase
5α-dihydrotestosterone
5α-reductase
DHT (17β-hydroxy-5α-androstan-3-one)
T,(17β-hydroxy-androst-4-en-3-one)
androgen
androst-4-ene-3-,17-dione)
androstenedione
metabolism
pituitary
testosterone

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology

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10.1016/0303-7207(80)90020-9

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title = "Androgen metabolism in rat anterior pituitary cells in culture",

abstract = "The metabolism in vitro of 4 androgens, namely testosterone, androstenedione, 5α-dihydrotestosterone and 3α-androstanediol has been studied in male and female rat anterior pituitary cells in primary culture. When testosterone was used as precursor, androstenedione, 5α-dihydrotestosterone and 3α-androstanediol were the main metabolites whereas androstenedione was mainly converted into testosterone, 5α-androstanedione, 5α-dihydrotestosterone and androsterone. Studies on the metabolism of 5α-dihydrotestosterone and 3α-androstanediol showed that these compounds were easily interconverted and were also significantly metabolized to 5α-androstanedione and androsterone. No aromatized compounds could be detected suggesting that androgen action in the pituitary cell occurs directly via the androgen receptor rather than through prior conversion into estrogens.",

keywords = "17β-dehydrogenase, 3α-Adiol, (3α,17β-dihydroxy-5α-androstane), 3α-Adiol, (3α,17β-dihydroxy-5α-androstane), androst-4-ene-3,17-dione), 3α-androstanediol, 3α-androstanediol, 3α-dehydrogenase, 3β-Adiol (3β,17β-dihydroxy-5αx-androstane), 3β-androstanediol, 3β-dehydrogenase, 5α-dihydrotestosterone, 5α-reductase, DHT (17β-hydroxy-5α-androstan-3-one), T,(17β-hydroxy-androst-4-en-3-one), androgen, androst-4-ene-3-,17-dione), androstenedione, metabolism, pituitary, testosterone",

author = "A. Belanger and Lena Lundell and P. Eneroth and Gustafsson, {J. A.}",

note = "Funding Information: Requests for reprints should be addressed to: A. Belanger, Department of Molecular Endocrinology, Le Centre Hospitalier de l{\textquoteright}Universit6 Laval, Quebec GlV 4G2 (Canada). * This study was supported by a grant from the Swedish Medical Research Council (No. 13X-2819). ** Scholar of the Conseil de la Recherche en Sante du Quibec. Present address: Department of Molecular Endocrinology, Le Centre Hospitalier de I{\textquoteright}Universit6 Laval, Quebec GlV 4G2 (Canada).",

year = "1980",

doi = "10.1016/0303-7207(80)90020-9",

language = "English (US)",

volume = "19",

pages = "175--181",

journal = "Molecular and cellular endocrinology",

issn = "0303-7207",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Androgen metabolism in rat anterior pituitary cells in culture

AU - Belanger, A.

AU - Lundell, Lena

AU - Eneroth, P.

AU - Gustafsson, J. A.

N1 - Funding Information: Requests for reprints should be addressed to: A. Belanger, Department of Molecular Endocrinology, Le Centre Hospitalier de l’Universit6 Laval, Quebec GlV 4G2 (Canada). * This study was supported by a grant from the Swedish Medical Research Council (No. 13X-2819). ** Scholar of the Conseil de la Recherche en Sante du Quibec. Present address: Department of Molecular Endocrinology, Le Centre Hospitalier de I’Universit6 Laval, Quebec GlV 4G2 (Canada).

PY - 1980

Y1 - 1980

N2 - The metabolism in vitro of 4 androgens, namely testosterone, androstenedione, 5α-dihydrotestosterone and 3α-androstanediol has been studied in male and female rat anterior pituitary cells in primary culture. When testosterone was used as precursor, androstenedione, 5α-dihydrotestosterone and 3α-androstanediol were the main metabolites whereas androstenedione was mainly converted into testosterone, 5α-androstanedione, 5α-dihydrotestosterone and androsterone. Studies on the metabolism of 5α-dihydrotestosterone and 3α-androstanediol showed that these compounds were easily interconverted and were also significantly metabolized to 5α-androstanedione and androsterone. No aromatized compounds could be detected suggesting that androgen action in the pituitary cell occurs directly via the androgen receptor rather than through prior conversion into estrogens.

AB - The metabolism in vitro of 4 androgens, namely testosterone, androstenedione, 5α-dihydrotestosterone and 3α-androstanediol has been studied in male and female rat anterior pituitary cells in primary culture. When testosterone was used as precursor, androstenedione, 5α-dihydrotestosterone and 3α-androstanediol were the main metabolites whereas androstenedione was mainly converted into testosterone, 5α-androstanedione, 5α-dihydrotestosterone and androsterone. Studies on the metabolism of 5α-dihydrotestosterone and 3α-androstanediol showed that these compounds were easily interconverted and were also significantly metabolized to 5α-androstanedione and androsterone. No aromatized compounds could be detected suggesting that androgen action in the pituitary cell occurs directly via the androgen receptor rather than through prior conversion into estrogens.

KW - 17β-dehydrogenase

KW - 3α-Adiol, (3α,17β-dihydroxy-5α-androstane)

KW - 3α-Adiol, (3α,17β-dihydroxy-5α-androstane), androst-4-ene-3,17-dione)

KW - 3α-androstanediol

KW - 3α-dehydrogenase

KW - 3β-Adiol (3β,17β-dihydroxy-5αx-androstane)

KW - 3β-androstanediol

KW - 3β-dehydrogenase

KW - 5α-dihydrotestosterone

KW - 5α-reductase

KW - DHT (17β-hydroxy-5α-androstan-3-one)

KW - T,(17β-hydroxy-androst-4-en-3-one)

KW - androgen

KW - androst-4-ene-3-,17-dione)

KW - androstenedione

KW - metabolism

KW - pituitary

KW - testosterone

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UR - http://www.scopus.com/inward/citedby.url?scp=0018958488&partnerID=8YFLogxK

U2 - 10.1016/0303-7207(80)90020-9

DO - 10.1016/0303-7207(80)90020-9

M3 - Article

C2 - 7409304

AN - SCOPUS:0018958488

SN - 0303-7207

VL - 19

SP - 175

EP - 181

JO - Molecular and cellular endocrinology

JF - Molecular and cellular endocrinology

IS - 2

ER -

Androgen metabolism in rat anterior pituitary cells in culture

Abstract

Keywords

ASJC Scopus subject areas

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