TY - JOUR
T1 - Analytical methods for nano-bio interface interactions
AU - Wang, Jing
AU - Quershi, Waseem Akthar
AU - Li, Yiye
AU - Xu, Jianxun
AU - Nie, Guangjun
PY - 2016/10/14
Y1 - 2016/10/14
N2 - Knowledge on the interactions between engineered nanomaterials (ENMs) and biological systems is critical both for the assessment of biological effects of ENMs and for the rational design of ENM-based products. However, probing the events that occur at the nano-bio interface remains extremely challenging due to their complex and dynamic nature. So far, the understanding of mechanisms underlying nano-bio interactions has been mainly limited by the lack of proper analytical techniques with sufficient sensitivity, selectivity and resolution for characterization of nano-bio interface events. Moreover, many classic bioanalytical methods are not suitable for direct measurement of nano-bio interface interactions. These have made establishing analytical methodologies for systematic and comprehensive study of nano-bio interface one of the most focused areas in nanobiology. In this review we have discussed some representative developments regarding analytical techniques for nano-bio interface characterization, including the improvements of traditional methods and the emergence of powerful new technologies. These developments have allowed ultrasensitive, real-time analysis of interactions between ENMs and biomolecules, transformations of ENMs in biological environment, and impacts of ENMs on living systems on molecular or cellular level.
AB - Knowledge on the interactions between engineered nanomaterials (ENMs) and biological systems is critical both for the assessment of biological effects of ENMs and for the rational design of ENM-based products. However, probing the events that occur at the nano-bio interface remains extremely challenging due to their complex and dynamic nature. So far, the understanding of mechanisms underlying nano-bio interactions has been mainly limited by the lack of proper analytical techniques with sufficient sensitivity, selectivity and resolution for characterization of nano-bio interface events. Moreover, many classic bioanalytical methods are not suitable for direct measurement of nano-bio interface interactions. These have made establishing analytical methodologies for systematic and comprehensive study of nano-bio interface one of the most focused areas in nanobiology. In this review we have discussed some representative developments regarding analytical techniques for nano-bio interface characterization, including the improvements of traditional methods and the emergence of powerful new technologies. These developments have allowed ultrasensitive, real-time analysis of interactions between ENMs and biomolecules, transformations of ENMs in biological environment, and impacts of ENMs on living systems on molecular or cellular level.
KW - analytical methods
KW - engineered nanomaterial
KW - nano-bio interaction
KW - nano-bio interface
KW - protein corona
KW - synchrotron radiation
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U2 - 10.1007/s11426-016-0340-1
DO - 10.1007/s11426-016-0340-1
M3 - Article
AN - SCOPUS:84991730011
SN - 1674-7291
SP - 1
EP - 12
JO - Science China Chemistry
JF - Science China Chemistry
ER -