Analysis of the role of 5' regulatory mutations in the activation of quiescent metallothionein genes after carcinogen treatment.

E. Aguilar-Cordova; R. M. Lebovitz; Michael W. Lieberman

Analysis of the role of 5' regulatory mutations in the activation of quiescent metallothionein genes after carcinogen treatment.

E. Aguilar-Cordova, R. M. Lebovitz, Michael W. Lieberman

Research output: Contribution to journal › Article › peer-review

Abstract

S49 are mouse thymic lymphoma cells which do not express the two closely linked mouse metallothionein (MT) genes; however, previous studies demonstrated that treatment of S49 cells with chemical carcinogens or ultraviolet irradiation can activate these quiescent genes. To determine if activation of MT-I or MT-II in these variants is a result of cis-acting mutations, we amplified and sequenced the immediate 5' regions of 19 cadmium resistant S49 variants: MT-I+/MT-II-, MT-I-/MT-II+, MT-I+/MT-II+, and MT-I-/MT-II-. None of the variants contained mutations in the analyzed regions. Thus, the observed changes in MT expression must result from mutations at other sites or from non-mutational mechanisms.

Original language	English (US)
Pages (from-to)	217-221
Number of pages	5
Journal	Gene expression
Volume	1
Issue number	3
State	Published - 1991

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

@article{94a3cb7c36be42c2a433bdc527fa6745,

title = "Analysis of the role of 5' regulatory mutations in the activation of quiescent metallothionein genes after carcinogen treatment.",

abstract = "S49 are mouse thymic lymphoma cells which do not express the two closely linked mouse metallothionein (MT) genes; however, previous studies demonstrated that treatment of S49 cells with chemical carcinogens or ultraviolet irradiation can activate these quiescent genes. To determine if activation of MT-I or MT-II in these variants is a result of cis-acting mutations, we amplified and sequenced the immediate 5' regions of 19 cadmium resistant S49 variants: MT-I+/MT-II-, MT-I-/MT-II+, MT-I+/MT-II+, and MT-I-/MT-II-. None of the variants contained mutations in the analyzed regions. Thus, the observed changes in MT expression must result from mutations at other sites or from non-mutational mechanisms.",

author = "E. Aguilar-Cordova and Lebovitz, \{R. M.\} and Lieberman, \{Michael W.\}",

note = "Copyright: This record is sourced from MEDLINE{\textregistered}/PubMed{\textregistered}, a database of the U.S. National Library of Medicine",

year = "1991",

language = "English (US)",

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pages = "217--221",

journal = "Gene expression",

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T1 - Analysis of the role of 5' regulatory mutations in the activation of quiescent metallothionein genes after carcinogen treatment.

AU - Aguilar-Cordova, E.

AU - Lebovitz, R. M.

AU - Lieberman, Michael W.

N1 - Copyright: This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

PY - 1991

Y1 - 1991

N2 - S49 are mouse thymic lymphoma cells which do not express the two closely linked mouse metallothionein (MT) genes; however, previous studies demonstrated that treatment of S49 cells with chemical carcinogens or ultraviolet irradiation can activate these quiescent genes. To determine if activation of MT-I or MT-II in these variants is a result of cis-acting mutations, we amplified and sequenced the immediate 5' regions of 19 cadmium resistant S49 variants: MT-I+/MT-II-, MT-I-/MT-II+, MT-I+/MT-II+, and MT-I-/MT-II-. None of the variants contained mutations in the analyzed regions. Thus, the observed changes in MT expression must result from mutations at other sites or from non-mutational mechanisms.

AB - S49 are mouse thymic lymphoma cells which do not express the two closely linked mouse metallothionein (MT) genes; however, previous studies demonstrated that treatment of S49 cells with chemical carcinogens or ultraviolet irradiation can activate these quiescent genes. To determine if activation of MT-I or MT-II in these variants is a result of cis-acting mutations, we amplified and sequenced the immediate 5' regions of 19 cadmium resistant S49 variants: MT-I+/MT-II-, MT-I-/MT-II+, MT-I+/MT-II+, and MT-I-/MT-II-. None of the variants contained mutations in the analyzed regions. Thus, the observed changes in MT expression must result from mutations at other sites or from non-mutational mechanisms.

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AN - SCOPUS:0026281392

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Analysis of the role of 5' regulatory mutations in the activation of quiescent metallothionein genes after carcinogen treatment.

Abstract

ASJC Scopus subject areas

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