Analysis of the role of 5' regulatory mutations in the activation of quiescent metallothionein genes after carcinogen treatment.

E. Aguilar-Cordova, R. M. Lebovitz, Michael W. Lieberman

Research output: Contribution to journalArticle

Abstract

S49 are mouse thymic lymphoma cells which do not express the two closely linked mouse metallothionein (MT) genes; however, previous studies demonstrated that treatment of S49 cells with chemical carcinogens or ultraviolet irradiation can activate these quiescent genes. To determine if activation of MT-I or MT-II in these variants is a result of cis-acting mutations, we amplified and sequenced the immediate 5' regions of 19 cadmium resistant S49 variants: MT-I+/MT-II-, MT-I-/MT-II+, MT-I+/MT-II+, and MT-I-/MT-II-. None of the variants contained mutations in the analyzed regions. Thus, the observed changes in MT expression must result from mutations at other sites or from non-mutational mechanisms.

Original languageEnglish (US)
Pages (from-to)217-221
Number of pages5
JournalGene expression
Volume1
Issue number3
StatePublished - 1991

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Analysis of the role of 5' regulatory mutations in the activation of quiescent metallothionein genes after carcinogen treatment.'. Together they form a unique fingerprint.

Cite this