TY - JOUR
T1 - Analysis of the major mercapturic acid pathway metabolites of benzo[a]pyrene found in rat urine by nano-electrospray mass spectrometry
AU - Yang, Yang
AU - Rafter, Joseph
AU - Gustafsson, Jan Åke
AU - Sjövall, Jan
AU - Griffiths, William J.
PY - 1998
Y1 - 1998
N2 - Nano-electrospray has been used in combination with high resolution and tandem mass spectrometry in the analysis of the major mercapturic acid pathway metabolites of benzo[a]pyrene (BP). Accurate mass measurements indicate that the [M-H]- ion of the major metabolite has a chemical formula C25H22NO6S, which corresponds to the deprotonated form of tetrahydro-trihydroxy-BP-S-N-acetylcysteine. Tandem mass spectrometry of this [M-H]- ion results in a collision induced dissociation spectrum identical to that of synthetic 7,8,9,10-tetrahydro-8,9,10-trihydroxy-BP-7-S-N-acetylcysteine.
AB - Nano-electrospray has been used in combination with high resolution and tandem mass spectrometry in the analysis of the major mercapturic acid pathway metabolites of benzo[a]pyrene (BP). Accurate mass measurements indicate that the [M-H]- ion of the major metabolite has a chemical formula C25H22NO6S, which corresponds to the deprotonated form of tetrahydro-trihydroxy-BP-S-N-acetylcysteine. Tandem mass spectrometry of this [M-H]- ion results in a collision induced dissociation spectrum identical to that of synthetic 7,8,9,10-tetrahydro-8,9,10-trihydroxy-BP-7-S-N-acetylcysteine.
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U2 - 10.1002/(SICI)1097-0231(19980430)12:8<465::AID-RCM180>3.0.CO;2-F
DO - 10.1002/(SICI)1097-0231(19980430)12:8<465::AID-RCM180>3.0.CO;2-F
M3 - Article
C2 - 9586234
AN - SCOPUS:0031916298
SN - 0951-4198
VL - 12
SP - 465
EP - 471
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
IS - 8
ER -