Analysis of the individual allergens of russian thistle pollen by an enzyme-linked immunoblotting technique

Jorge Gomez; Lyndon E. Mansfield; Robert W. Frederick; Eppie D. Rael

doi:10.3109/02770908909073256

Analysis of the individual allergens of russian thistle pollen by an enzyme-linked immunoblotting technique

Jorge Gomez, Lyndon E. Mansfield, Robert W. Frederick, Eppie D. Rael

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Russian thistle pollen extract was analyzed by im-munoblots of isoelectic focused and SDS-PAGE gels. Twenty distinct protein bands were recognized by human IgE- and IgG-specific antibodies in the immunoblot from the SDS-PAGE gel. Molecular weights of these allergens ranged from 12.2 kD to 85 kD. Seventeen bands were detected on isoelectric focusing immunoblots with pi from 3.95 to 7.70. Allergic subjects had differing individual patterns of protein band recognition. Immunoblot techniques provide detailed evaluations of the response of allergy subjects to components of crude natural allergens.

Original language	English (US)
Pages (from-to)	243-250
Number of pages	8
Journal	Journal of Asthma
Volume	26
Issue number	4
DOIs	https://doi.org/10.3109/02770908909073256
State	Published - 1989

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Immunology and Allergy
Pulmonary and Respiratory Medicine

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title = "Analysis of the individual allergens of russian thistle pollen by an enzyme-linked immunoblotting technique",

abstract = "Russian thistle pollen extract was analyzed by im-munoblots of isoelectic focused and SDS-PAGE gels. Twenty distinct protein bands were recognized by human IgE- and IgG-specific antibodies in the immunoblot from the SDS-PAGE gel. Molecular weights of these allergens ranged from 12.2 kD to 85 kD. Seventeen bands were detected on isoelectric focusing immunoblots with pi from 3.95 to 7.70. Allergic subjects had differing individual patterns of protein band recognition. Immunoblot techniques provide detailed evaluations of the response of allergy subjects to components of crude natural allergens.",

author = "Jorge Gomez and Mansfield, {Lyndon E.} and Frederick, {Robert W.} and Rael, {Eppie D.}",

note = "Funding Information: University of Texas El Paso, Texas Tech University Health Sciences Center William Beaumont Army Medical Center Paso Institute for Medical Research and Development El Paso, Texas Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1989",

doi = "10.3109/02770908909073256",

language = "English (US)",

volume = "26",

pages = "243--250",

journal = "Journal of Asthma",

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T1 - Analysis of the individual allergens of russian thistle pollen by an enzyme-linked immunoblotting technique

AU - Gomez, Jorge

AU - Mansfield, Lyndon E.

AU - Frederick, Robert W.

AU - Rael, Eppie D.

N1 - Funding Information: University of Texas El Paso, Texas Tech University Health Sciences Center William Beaumont Army Medical Center Paso Institute for Medical Research and Development El Paso, Texas Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1989

Y1 - 1989

N2 - Russian thistle pollen extract was analyzed by im-munoblots of isoelectic focused and SDS-PAGE gels. Twenty distinct protein bands were recognized by human IgE- and IgG-specific antibodies in the immunoblot from the SDS-PAGE gel. Molecular weights of these allergens ranged from 12.2 kD to 85 kD. Seventeen bands were detected on isoelectric focusing immunoblots with pi from 3.95 to 7.70. Allergic subjects had differing individual patterns of protein band recognition. Immunoblot techniques provide detailed evaluations of the response of allergy subjects to components of crude natural allergens.

AB - Russian thistle pollen extract was analyzed by im-munoblots of isoelectic focused and SDS-PAGE gels. Twenty distinct protein bands were recognized by human IgE- and IgG-specific antibodies in the immunoblot from the SDS-PAGE gel. Molecular weights of these allergens ranged from 12.2 kD to 85 kD. Seventeen bands were detected on isoelectric focusing immunoblots with pi from 3.95 to 7.70. Allergic subjects had differing individual patterns of protein band recognition. Immunoblot techniques provide detailed evaluations of the response of allergy subjects to components of crude natural allergens.

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ER -

Analysis of the individual allergens of russian thistle pollen by an enzyme-linked immunoblotting technique

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