Analysis of the carbohydrate composition of glycoproteins by high-performance liquid chromatography

Eugene Y.J. Kang, Richard D. Coleman, Henry J. Pownall, Antonio Gotto, Chao Yuh Yang

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A procedure for rapid and sensitive analysis of carbohydrate in glycoproteins is described. After methanolysis and benzoylation of the monosaccharides and carbohydrates of a glycoprotein, the derivatized sugars were analyzed by reverse-phase high-performance chromatography using a Vydac C18 stationary phase and a mobile phase composed of a water/acetonitrile gradient. The advantages of this procedure over previously described methods are (1) the simple binary solvent system which is used requires no buffering salts and (2) separate sets of peaks from individual sugars obviate the usual need to reacetylate sugar amino groups.

Original languageEnglish (US)
Pages (from-to)31-35
Number of pages5
JournalJournal of Protein Chemistry
Volume9
Issue number1
DOIs
StatePublished - Feb 1990

Keywords

  • carbohydrates
  • carjohydrate compositions
  • glycopeptides
  • glycoprotuyns
  • HPLC

ASJC Scopus subject areas

  • Biochemistry

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