Analysis of the carbohydrate composition of glycoproteins by high-performance liquid chromatography

Eugene Y.J. Kang; Richard D. Coleman; Henry J. Pownall; Antonio Gotto; Chao Yuh Yang

doi:10.1007/BF01024981

Analysis of the carbohydrate composition of glycoproteins by high-performance liquid chromatography

Eugene Y.J. Kang, Richard D. Coleman, Henry J. Pownall, Antonio Gotto, Chao Yuh Yang

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14 Scopus citations

Abstract

A procedure for rapid and sensitive analysis of carbohydrate in glycoproteins is described. After methanolysis and benzoylation of the monosaccharides and carbohydrates of a glycoprotein, the derivatized sugars were analyzed by reverse-phase high-performance chromatography using a Vydac C₁₈ stationary phase and a mobile phase composed of a water/acetonitrile gradient. The advantages of this procedure over previously described methods are (1) the simple binary solvent system which is used requires no buffering salts and (2) separate sets of peaks from individual sugars obviate the usual need to reacetylate sugar amino groups.

Original language	English (US)
Pages (from-to)	31-35
Number of pages	5
Journal	Journal of Protein Chemistry
Volume	9
Issue number	1
DOIs	https://doi.org/10.1007/BF01024981
State	Published - Feb 1990

Keywords

carbohydrates
carjohydrate compositions
glycopeptides
glycoprotuyns
HPLC

ASJC Scopus subject areas

Biochemistry

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10.1007/BF01024981

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title = "Analysis of the carbohydrate composition of glycoproteins by high-performance liquid chromatography",

abstract = "A procedure for rapid and sensitive analysis of carbohydrate in glycoproteins is described. After methanolysis and benzoylation of the monosaccharides and carbohydrates of a glycoprotein, the derivatized sugars were analyzed by reverse-phase high-performance chromatography using a Vydac C18 stationary phase and a mobile phase composed of a water/acetonitrile gradient. The advantages of this procedure over previously described methods are (1) the simple binary solvent system which is used requires no buffering salts and (2) separate sets of peaks from individual sugars obviate the usual need to reacetylate sugar amino groups.",

keywords = "carbohydrates, carjohydrate compositions, glycopeptides, glycoprotuyns, HPLC",

author = "Kang, {Eugene Y.J.} and Coleman, {Richard D.} and Pownall, {Henry J.} and Antonio Gotto and Yang, {Chao Yuh}",

year = "1990",

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doi = "10.1007/BF01024981",

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T1 - Analysis of the carbohydrate composition of glycoproteins by high-performance liquid chromatography

AU - Kang, Eugene Y.J.

AU - Coleman, Richard D.

AU - Pownall, Henry J.

AU - Gotto, Antonio

AU - Yang, Chao Yuh

PY - 1990/2

Y1 - 1990/2

N2 - A procedure for rapid and sensitive analysis of carbohydrate in glycoproteins is described. After methanolysis and benzoylation of the monosaccharides and carbohydrates of a glycoprotein, the derivatized sugars were analyzed by reverse-phase high-performance chromatography using a Vydac C18 stationary phase and a mobile phase composed of a water/acetonitrile gradient. The advantages of this procedure over previously described methods are (1) the simple binary solvent system which is used requires no buffering salts and (2) separate sets of peaks from individual sugars obviate the usual need to reacetylate sugar amino groups.

AB - A procedure for rapid and sensitive analysis of carbohydrate in glycoproteins is described. After methanolysis and benzoylation of the monosaccharides and carbohydrates of a glycoprotein, the derivatized sugars were analyzed by reverse-phase high-performance chromatography using a Vydac C18 stationary phase and a mobile phase composed of a water/acetonitrile gradient. The advantages of this procedure over previously described methods are (1) the simple binary solvent system which is used requires no buffering salts and (2) separate sets of peaks from individual sugars obviate the usual need to reacetylate sugar amino groups.

KW - carbohydrates

KW - carjohydrate compositions

KW - glycopeptides

KW - glycoprotuyns

KW - HPLC

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JO - Journal of Protein Chemistry

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ER -

Analysis of the carbohydrate composition of glycoproteins by high-performance liquid chromatography

Abstract

Keywords

ASJC Scopus subject areas

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