Analysis of regulatory sequences in androgen-responsive genes

Malcolm G. Parker; Paul Webb; John S. Mills; Maurice Needham; Roger White

doi:10.1016/0022-4731(88)90075-1

Analysis of regulatory sequences in androgen-responsive genes

Malcolm G. Parker, Paul Webb, John S. Mills, Maurice Needham, Roger White

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We have analysed the effect of androgens on the activity of promoters from MMTV, and the rat prostate C3 (1) and mouse secretory protease inhibitor genes. MMTV promoter activity was stimulated by testosterone as well as progesterone and dexamethasone but not by oestradiol. Deletion analysis indicated that the three steroids acted through DNA sequences between nucleotides -201 and -69 upstream of the MMTV cap site. In contrast, the promoters for the C3(1) gene and the protease inhibitor gene were unaffected by testosterone in a number of cell types, including prostate cells, despite the fact that the MMTV promoter was stimulated in such cells.

Original language	English (US)
Pages (from-to)	47-51
Number of pages	5
Journal	Journal of Steroid Biochemistry
Volume	30
Issue number	1-6
DOIs	https://doi.org/10.1016/0022-4731(88)90075-1
State	Published - Jan 1 1988

ASJC Scopus subject areas

Biochemistry
Endocrinology

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10.1016/0022-4731(88)90075-1

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AU - Needham, Maurice

AU - White, Roger

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N2 - We have analysed the effect of androgens on the activity of promoters from MMTV, and the rat prostate C3 (1) and mouse secretory protease inhibitor genes. MMTV promoter activity was stimulated by testosterone as well as progesterone and dexamethasone but not by oestradiol. Deletion analysis indicated that the three steroids acted through DNA sequences between nucleotides -201 and -69 upstream of the MMTV cap site. In contrast, the promoters for the C3(1) gene and the protease inhibitor gene were unaffected by testosterone in a number of cell types, including prostate cells, despite the fact that the MMTV promoter was stimulated in such cells.

AB - We have analysed the effect of androgens on the activity of promoters from MMTV, and the rat prostate C3 (1) and mouse secretory protease inhibitor genes. MMTV promoter activity was stimulated by testosterone as well as progesterone and dexamethasone but not by oestradiol. Deletion analysis indicated that the three steroids acted through DNA sequences between nucleotides -201 and -69 upstream of the MMTV cap site. In contrast, the promoters for the C3(1) gene and the protease inhibitor gene were unaffected by testosterone in a number of cell types, including prostate cells, despite the fact that the MMTV promoter was stimulated in such cells.

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Analysis of regulatory sequences in androgen-responsive genes

Abstract

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