Analysis of nonlinear responses of adherent epithelial cells probed by magnetic bead twisting: A finite element model based on a homogenization approach

Jacques Ohayon; Philippe Tracqui; Redouane Fodil; Sophie Féréol; Valérie M. Laurent; Emmanuelle Planus; Daniel Isabey

doi:10.1115/1.1824136

Analysis of nonlinear responses of adherent epithelial cells probed by magnetic bead twisting: A finite element model based on a homogenization approach

Jacques Ohayon, Philippe Tracqui, Redouane Fodil, Sophie Féréol, Valérie M. Laurent, Emmanuelle Planus, Daniel Isabey

Research output: Contribution to journal › Article

37 Scopus citations

Abstract

An original homogenization method was used to analyze the nonlinear elastic properties of epithelial cells probed by magnetic twisting cytometry. In this approach, the apparent rigidity of a cell with nonlinear mechanical properties is deduced from the mechanical response of the entire population of adherent cells. The proposed hyperelastic cell model successfully accounts for the variability in probe-cell geometrical features, and the influence of the cell-substrate adhesion. Spatially distributed local secant elastic moduli had amplitudes ranging from 10 to 400 Pa. The nonlinear elastic behavior of cells may contribute to the wide differences in published results regarding cell elasticity moduli.

Original language	English (US)
Pages (from-to)	685-698
Number of pages	14
Journal	Journal of Biomechanical Engineering
Volume	126
Issue number	6
DOIs	https://doi.org/10.1115/1.1824136
State	Published - Dec 2004

Keywords

Cell adhesion
Contact mechanics
Cytomechanical model
Finite element method
Homogenization
Hyperelasticity
Magnetocytometry

ASJC Scopus subject areas

Biomedical Engineering
Physiology (medical)

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10.1115/1.1824136

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Analysis of nonlinear responses of adherent epithelial cells probed by magnetic bead twisting : A finite element model based on a homogenization approach. / Ohayon, Jacques; Tracqui, Philippe; Fodil, Redouane; Féréol, Sophie; Laurent, Valérie M.; Planus, Emmanuelle; Isabey, Daniel.

In: Journal of Biomechanical Engineering, Vol. 126, No. 6, 12.2004, p. 685-698.

Research output: Contribution to journal › Article

Ohayon, Jacques ; Tracqui, Philippe ; Fodil, Redouane ; Féréol, Sophie ; Laurent, Valérie M. ; Planus, Emmanuelle ; Isabey, Daniel. / Analysis of nonlinear responses of adherent epithelial cells probed by magnetic bead twisting : A finite element model based on a homogenization approach. In: Journal of Biomechanical Engineering. 2004 ; Vol. 126, No. 6. pp. 685-698.

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