TY - JOUR
T1 - Analysis of aryl hydrocarbon hydroxylase activity in human lung tissue, pulmonary macrophages, and blood lymphocytes
AU - McLemore, Theodore L.
AU - Martin, R. Russell
AU - Springer, Robert R.
AU - Wray, Nelda
AU - Toppell, Kenneth L.
AU - Pickard, Laurens R.
AU - Mattox, Kenneth L.
AU - Guinn, Gene A.
AU - Cantrell, Elroy T.
AU - Busbee, David L.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1978/6
Y1 - 1978/6
N2 - Aryl hydrocarbon hydroxylase (AHH) activity was measured fluorometrically in surgically-excised fresh lung tissue, pulmonary alveolar macrophages (PAMs), and peripheral blood lymphocytes from 14 cigarette smokers (7 with and 7 without primary lung cancer). Levels of AHH in fresh PAMs and AHH inducibility (expressed as fold-induction) in cultured, mitogen-stimulated lymphocytes from individual noncancer patients correlated well (r = 0.975, p < .001). For individual lung cancer patients, however, these values were dissociated (linear regression not appropriate for this set of values). Levels of AHH in fresh lung tissue and fold-induction ratios in cultured lymphocytes from individual noncancer patients also exhibited a positive correlation (r = 0.976, p < .001), while values for individual lung cancer patients did not (r = 0.007, p = 0.987). A close agreement was noted for AHH in fresh lung tissue and fresh PAMs from individual noncancer patients (r = 0.984, p < .001), while these values are weakly correlated for lung cancer patients (r = 0.658, p < .11). When AHH activity in fresh PAMs, in fresh lung tissue, and AHH inducibility in cultured lymphocytes were simultaneously compared, an excellent relationship was observed for values for all 3 tissues for individual noncancer patients (r = 0.987, p < .001). However, AHH levels in these 3 tissues from individual lung cancer patients were not correlated (r = 0.701, p > .25). These results indicate similar capacity for AHH induction is present in fresh lung tissue, fresh PAMs, and cultured mitogen-stimulated lymphocytes from cigarette smokers without evidence of lung cancer, but AHH values are not positively correlated with similar tissues from individual lung cancer patients.
AB - Aryl hydrocarbon hydroxylase (AHH) activity was measured fluorometrically in surgically-excised fresh lung tissue, pulmonary alveolar macrophages (PAMs), and peripheral blood lymphocytes from 14 cigarette smokers (7 with and 7 without primary lung cancer). Levels of AHH in fresh PAMs and AHH inducibility (expressed as fold-induction) in cultured, mitogen-stimulated lymphocytes from individual noncancer patients correlated well (r = 0.975, p < .001). For individual lung cancer patients, however, these values were dissociated (linear regression not appropriate for this set of values). Levels of AHH in fresh lung tissue and fold-induction ratios in cultured lymphocytes from individual noncancer patients also exhibited a positive correlation (r = 0.976, p < .001), while values for individual lung cancer patients did not (r = 0.007, p = 0.987). A close agreement was noted for AHH in fresh lung tissue and fresh PAMs from individual noncancer patients (r = 0.984, p < .001), while these values are weakly correlated for lung cancer patients (r = 0.658, p < .11). When AHH activity in fresh PAMs, in fresh lung tissue, and AHH inducibility in cultured lymphocytes were simultaneously compared, an excellent relationship was observed for values for all 3 tissues for individual noncancer patients (r = 0.987, p < .001). However, AHH levels in these 3 tissues from individual lung cancer patients were not correlated (r = 0.701, p > .25). These results indicate similar capacity for AHH induction is present in fresh lung tissue, fresh PAMs, and cultured mitogen-stimulated lymphocytes from cigarette smokers without evidence of lung cancer, but AHH values are not positively correlated with similar tissues from individual lung cancer patients.
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U2 - 10.1002/1097-0142(197806)41:6<2292::AID-CNCR2820410630>3.0.CO;2-2
DO - 10.1002/1097-0142(197806)41:6<2292::AID-CNCR2820410630>3.0.CO;2-2
M3 - Article
C2 - 657093
AN - SCOPUS:0018143464
SN - 0008-543X
VL - 41
SP - 2292
EP - 2300
JO - Cancer
JF - Cancer
IS - 6
ER -