Analysis of a gene cluster of Enterococcus faecalis involved in polysaccharide biosynthesis

Yi Xu; Kavindra V. Singh; Xiang Qin; Barbara E. Murray; George M. Weinstock

doi:10.1128/IAI.68.2.815-823.2000

Analysis of a gene cluster of Enterococcus faecalis involved in polysaccharide biosynthesis

Yi Xu, Kavindra V. Singh, Xiang Qin, Barbara E. Murray, George M. Weinstock

Research output: Contribution to journal › Article › peer-review

84 Scopus citations

Abstract

Previously, we described a gene cluster of Enterococcus faecalis OG1RF that produced an antigenic polysaccharide when cloned in Escherichia coli. The polysaccharide antigen was not detectable in E. faecalis strains, however. Here, we show by reverse transcriptase-PCR that the 16 genes in this region are transcribed in OG1RF. Gene disruption of orfde4, encoding a putative glycosyl transferase, and orfde6, a putative dTDP-rhamnose biosynthesis gene, generated two OG1RF mutants. The mutants showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. In addition, two mucoid E. faecalis isolates from patients with chronic urinary tract infections were found to produce the polysaccharide antigen.

Original language	English (US)
Pages (from-to)	815-823
Number of pages	9
Journal	Infection and Immunity
Volume	68
Issue number	2
DOIs	https://doi.org/10.1128/IAI.68.2.815-823.2000
State	Published - Feb 2000

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Infectious Diseases

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10.1128/IAI.68.2.815-823.2000

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title = "Analysis of a gene cluster of Enterococcus faecalis involved in polysaccharide biosynthesis",

abstract = "Previously, we described a gene cluster of Enterococcus faecalis OG1RF that produced an antigenic polysaccharide when cloned in Escherichia coli. The polysaccharide antigen was not detectable in E. faecalis strains, however. Here, we show by reverse transcriptase-PCR that the 16 genes in this region are transcribed in OG1RF. Gene disruption of orfde4, encoding a putative glycosyl transferase, and orfde6, a putative dTDP-rhamnose biosynthesis gene, generated two OG1RF mutants. The mutants showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. In addition, two mucoid E. faecalis isolates from patients with chronic urinary tract infections were found to produce the polysaccharide antigen.",

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AU - Weinstock, George M.

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AB - Previously, we described a gene cluster of Enterococcus faecalis OG1RF that produced an antigenic polysaccharide when cloned in Escherichia coli. The polysaccharide antigen was not detectable in E. faecalis strains, however. Here, we show by reverse transcriptase-PCR that the 16 genes in this region are transcribed in OG1RF. Gene disruption of orfde4, encoding a putative glycosyl transferase, and orfde6, a putative dTDP-rhamnose biosynthesis gene, generated two OG1RF mutants. The mutants showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. In addition, two mucoid E. faecalis isolates from patients with chronic urinary tract infections were found to produce the polysaccharide antigen.

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Analysis of a gene cluster of Enterococcus faecalis involved in polysaccharide biosynthesis

Abstract

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