Analyses of programmed cell death in dendritic cells

Min Chen, Lily Huang, Jin Wang

Research output: Contribution to journalArticle

Abstract

Dendritic cells (DCs) are professional antigen-presenting cells that can regulate both innate and adaptive immune responses. Programmed cell death of DCs plays an important role in maintaining the homeostasis of DCs and in the regulation of immune responses. Methods to measure the rate of spontaneous and T cell-mediated cell death of DCs are described here. These procedures can be adapted to study the -induction of dendritic cell death in different settings of immune responses.

Original languageEnglish (US)
Pages (from-to)51-63
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Volume979
DOIs
StatePublished - 2013

Keywords

  • Adoptive Transfer
  • Animals
  • Apoptosis
  • Bone Marrow Cells
  • Bromodeoxyuridine
  • CD4-Positive T-Lymphocytes
  • CD8-Positive T-Lymphocytes
  • Cell Separation
  • Cytological Techniques
  • Dendritic Cells
  • Lymphocyte Activation
  • Mice
  • Peptides
  • Spleen
  • Journal Article
  • Research Support, N.I.H., Extramural

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