Analyses of programmed cell death in dendritic cells

Min Chen; Lily Huang; Jin Wang

doi:10.1007/978-1-62703-290-2_6

Analyses of programmed cell death in dendritic cells

Min Chen, Lily Huang, Jin Wang

Research Institute

Research output: Contribution to journal › Article

Abstract

Dendritic cells (DCs) are professional antigen-presenting cells that can regulate both innate and adaptive immune responses. Programmed cell death of DCs plays an important role in maintaining the homeostasis of DCs and in the regulation of immune responses. Methods to measure the rate of spontaneous and T cell-mediated cell death of DCs are described here. These procedures can be adapted to study the -induction of dendritic cell death in different settings of immune responses.

Original language	English (US)
Pages (from-to)	51-63
Number of pages	13
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	979
DOIs	https://doi.org/10.1007/978-1-62703-290-2_6
State	Published - 2013

Keywords

Adoptive Transfer
Animals
Apoptosis
Bone Marrow Cells
Bromodeoxyuridine
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Cell Separation
Cytological Techniques
Dendritic Cells
Lymphocyte Activation
Mice
Peptides
Spleen
Journal Article
Research Support, N.I.H., Extramural

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10.1007/978-1-62703-290-2_6

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title = "Analyses of programmed cell death in dendritic cells",

abstract = "Dendritic cells (DCs) are professional antigen-presenting cells that can regulate both innate and adaptive immune responses. Programmed cell death of DCs plays an important role in maintaining the homeostasis of DCs and in the regulation of immune responses. Methods to measure the rate of spontaneous and T cell-mediated cell death of DCs are described here. These procedures can be adapted to study the -induction of dendritic cell death in different settings of immune responses.",

keywords = "Adoptive Transfer, Animals, Apoptosis, Bone Marrow Cells, Bromodeoxyuridine, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cell Separation, Cytological Techniques, Dendritic Cells, Lymphocyte Activation, Mice, Peptides, Spleen, Journal Article, Research Support, N.I.H., Extramural",

author = "Min Chen and Lily Huang and Jin Wang",

year = "2013",

doi = "10.1007/978-1-62703-290-2_6",

language = "English (US)",

volume = "979",

pages = "51--63",

journal = "Methods in molecular biology (Clifton, N.J.)",

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TY - JOUR

T1 - Analyses of programmed cell death in dendritic cells

AU - Chen, Min

AU - Huang, Lily

AU - Wang, Jin

PY - 2013

Y1 - 2013

N2 - Dendritic cells (DCs) are professional antigen-presenting cells that can regulate both innate and adaptive immune responses. Programmed cell death of DCs plays an important role in maintaining the homeostasis of DCs and in the regulation of immune responses. Methods to measure the rate of spontaneous and T cell-mediated cell death of DCs are described here. These procedures can be adapted to study the -induction of dendritic cell death in different settings of immune responses.

AB - Dendritic cells (DCs) are professional antigen-presenting cells that can regulate both innate and adaptive immune responses. Programmed cell death of DCs plays an important role in maintaining the homeostasis of DCs and in the regulation of immune responses. Methods to measure the rate of spontaneous and T cell-mediated cell death of DCs are described here. These procedures can be adapted to study the -induction of dendritic cell death in different settings of immune responses.

KW - Adoptive Transfer

KW - Animals

KW - Apoptosis

KW - Bone Marrow Cells

KW - Bromodeoxyuridine

KW - CD4-Positive T-Lymphocytes

KW - CD8-Positive T-Lymphocytes

KW - Cell Separation

KW - Cytological Techniques

KW - Dendritic Cells

KW - Lymphocyte Activation

KW - Mice

KW - Peptides

KW - Spleen

KW - Journal Article

KW - Research Support, N.I.H., Extramural

U2 - 10.1007/978-1-62703-290-2_6

DO - 10.1007/978-1-62703-290-2_6

M3 - Article

C2 - 23397388

SN - 1064-3745

VL - 979

SP - 51

EP - 63

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Analyses of programmed cell death in dendritic cells

Abstract

Keywords

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