An ultra pH-sensitive and aptamer-equipped nanoscale drug-delivery system for selective killing of tumor cells

Nianxi Zhao, Jian You, Zihua Zeng, Chun Li, Youli Zu

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Nanotechnology has often been applied in the development of targeted drug-delivery systems for the treatment of cancer. An ideal nanoscale system for drug delivery should be able to selectively deliver and rapidly release the carried therapeutic drug(s) in cancer cells and, more importantly, not react to off-target cells so as to eliminate unwanted toxicity on normal tissues. To reach this goal, a selective chemotherapeutic is formulated using a hollow gold nanosphere (HAuNS) equipped with a biomarker-specific aptamer (Apt), and loaded with the chemotherapy drug doxorubicin (DOX). The formed Apt-HAuNS-Dox, approximately 42 nm in diameter, specifically binds to lymphoma tumor cells and does not react to control cells that do not express the biomarker. Through aptamer-mediated selective cell binding, the Apt-HAuNS-Dox is internalized exclusively into the targeted tumor cells, and then released the DOX intracellularly. Of note, although the formed Apt-HAuNS-Dox is stable under normal biological conditions (pH 7.4), it appears ultrasensitive to pH change and rapidly releases 80% of the loaded DOX within 2 h at pH 5.0, a condition seen in cell lysosomes. Functional assays using cell mixtures show that the Apt-HAuNS-Dox selectively kills lymphoma tumor cells, but has no effect on the growth of the off-target cells in the same cultures, indicating that this ultra pH-sensitive Apt-HAuNS-Dox can selectively treat cancer through specific aptamer guidance, and will have minimal side effects on normal tissue. Using aptamer technology, an innovative ultra pH-sensitive nanoscale drug-delivery system is developed that selectively kills lymphoma tumor cells with no effects on off-target cells. The successful in vitro tests reported here represent an important step in the development of next-generation targeted cancer therapeutics.

Original languageEnglish (US)
Pages (from-to)3477-3484
Number of pages8
JournalSmall
Volume9
Issue number20
DOIs
StatePublished - Oct 25 2013

Keywords

  • aptamers
  • drug delivery
  • hollow gold nanospheres
  • pH sensitive
  • targeted therapies

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

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