An investigation on the nature of the peptide at m/z 904, overexpressed in plasma of patients with colorectal cancer and familial adenomatous polyposis

Roberta Seraglia, Laura Molin, Loris Tonidandel, Salvatore Pucciarelli, Marco Agostini, Emanuele Damiano Urso, Chiara Bedin, Michele Quaia, Donato Nitti, Pietro Traldi

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

In an investigation devoted to the search for plasma markers for colorectal cancer (CRC), carried out by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, a series of overexpressed peptides were identified in the plasma of patients. Among them the peptide with molecular weight 903 Da was the most abundant one, with a mean ± (SD) relative abundance of 37 ± 17% and a frequency over 60%. Interestingly, also in plasma samples of ten subjects affected by familial adenomatous polyposis (FAP), the peptide with molecular weight 903 was overexpressed. In this investigation, MALDI/MS/MS experiments were carried out on the ion at m/z 904 detected in the MALDI mass spectra of CRC and FAP patients. The data analysis by SwissProt.2007.01.09 indicates that this peptide is due to the sequence RPPGFSPF, found in the kininogen-1 precursor, which is an a-2-thiol proteinase inhibitor. In the case of subjects affected by a particular FAP syndrome, the MALDI/MS/MS spectra were quite different from those obtained from CRC and FAP patients. In fact, two sequences have been evidenced: RPPGFSPF belonging to kininogen-1 precursor, and PRKSSSSR belonging to Forkhead box protein 01A.

Original languageEnglish (US)
Pages (from-to)1606-1612
Number of pages7
JournalJournal of Mass Spectrometry
Volume42
Issue number12
DOIs
StatePublished - Dec 1 2007

Keywords

  • Colorectal cancer
  • Familial adenomatous polyposis
  • MALDI
  • Plasma
  • TOF-TOF

ASJC Scopus subject areas

  • Organic Chemistry
  • Spectroscopy
  • Biophysics

Fingerprint Dive into the research topics of 'An investigation on the nature of the peptide at m/z 904, overexpressed in plasma of patients with colorectal cancer and familial adenomatous polyposis'. Together they form a unique fingerprint.

Cite this