An inducible alpha-synuclein expressing neuronal cell line model for Parkinson's disease

Velmarini Vasquez, Joy Mitra, George Perry, K. S. Rao, Muralidhar L. Hegde

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Altered expression of α-synuclein is linked to Parkinson's disease (PD). A major challenge to explore how the increased α-synuclein affect neurotoxicity is the lack of a suitable human neuronal cell model that mimics this scenario. Its expression in neural precursors affects their differentiation process, in addition to the neuronal adaptability and variability in maintaining a constant level of expression across passages. Here, we describe an SH-SY5Y line harboring Tet-ON SNCA cDNA cassette that allows for induction of controlled α-synuclein expression after neuronal differentiation, which can be an important tool for PD research.

Original languageEnglish (US)
Pages (from-to)453-460
Number of pages8
JournalJournal of Alzheimer's Disease
Volume66
Issue number2
DOIs
StatePublished - 2018

Keywords

  • Parkinson's disease
  • dopaminergic neurons
  • inducible α-synuclein expressing neuronal model for PD
  • α-synuclein

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Psychology
  • Geriatrics and Gerontology
  • Psychiatry and Mental health

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