TY - JOUR
T1 - An IKKα-Nucleophosmin axis utilizes inflammatory signaling to promote genome integrity
AU - Xia, Xiaojun
AU - Liu, Shuang
AU - Xiao, Zuoxiang
AU - Zhu, Feng
AU - Song, Na Young
AU - Zhou, Ming
AU - Liu, Bigang
AU - Shen, Jianjun
AU - Nagashima, Kunio
AU - Veenstra, Timothy D.
AU - Burkett, Sandra
AU - Datla, Mahesh
AU - Willette-Brown, Jami
AU - Shen, Haifa
AU - Hu, Yinling
N1 - Funding Information:
We thank D. Butcher, I. Gimenez-Conti, and N.W. Otto for the immunohistochemical staining. This work was supported by funding from the National Cancer Institute (CA-102510, ZIA BC011212, and ZIA BC 011391 to Y.H.).
PY - 2013/12/12
Y1 - 2013/12/12
N2 - The inflammatory microenvironment promotes skin tumorigenesis. However, the mechanisms by which cells protect themselves from inflammatory signals are unknown. Downregulation of IKKα promotes skin tumor progression from papillomas to squamous cell carcinomas, which is frequently accompanied bygenomic instability, including aneuploid chromosomes and extra centrosomes. In this study, we found that IKKα promoted oligomerization of nucleophosmin (NPM), a negative centrosome duplication regulator, which further enhanced NPM and centrosome association, inhibited centrosome amplification, and maintained genome integrity. Levels of NPM hexamers and IKKα were conversely associated with skin tumor progression. Importantly, proinflammatory cytokine-induced IKKα activation promoted the formation of NPM oligomers and reduced centrosome numbers in mouse and human cells, whereas kinase-dead IKKα blocked this connection. Therefore, our findings suggest a mechanism in which an IKKα-NPM axis may use inflammatory signals to suppress centrosome amplification, promote genomic integrity, and prevent tumor progression.
AB - The inflammatory microenvironment promotes skin tumorigenesis. However, the mechanisms by which cells protect themselves from inflammatory signals are unknown. Downregulation of IKKα promotes skin tumor progression from papillomas to squamous cell carcinomas, which is frequently accompanied bygenomic instability, including aneuploid chromosomes and extra centrosomes. In this study, we found that IKKα promoted oligomerization of nucleophosmin (NPM), a negative centrosome duplication regulator, which further enhanced NPM and centrosome association, inhibited centrosome amplification, and maintained genome integrity. Levels of NPM hexamers and IKKα were conversely associated with skin tumor progression. Importantly, proinflammatory cytokine-induced IKKα activation promoted the formation of NPM oligomers and reduced centrosome numbers in mouse and human cells, whereas kinase-dead IKKα blocked this connection. Therefore, our findings suggest a mechanism in which an IKKα-NPM axis may use inflammatory signals to suppress centrosome amplification, promote genomic integrity, and prevent tumor progression.
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U2 - 10.1016/j.celrep.2013.10.046
DO - 10.1016/j.celrep.2013.10.046
M3 - Article
C2 - 24290756
AN - SCOPUS:84890175189
SN - 2211-1247
VL - 5
SP - 1243
EP - 1255
JO - Cell Reports
JF - Cell Reports
IS - 5
ER -