An electrochemical detector array to study cell biology on the nanoscale

Andrew F. Dias, Gregor Dernick, Vicente Valero, Ming G. Yong, Conrad D. James, Harold G. Craighead, Manfred Lindau

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Nanobiotechnology is a field that utilizes the techniques of nano- and microfabrication to study biosystems or to use biological material and principles to build new devices. As an example we discuss the development of a nanofabricated electrochemical detector array that reveals the spatio-temporal dynamics of exocytosis in single chromaffin cells. In a quantal release event a single vesicle fuses with the plasma membrane releasing its contents through the fusion pore. The time-resolved amperometric currents measured by the individual electrodes detecting different fractions of the released molecules allow determination of the time course as well as localization of quantal events. Such a device may be Applicable to study the correlation of exocytotic events with signalling events that could be simultaneously monitored by fluorescence microscopy.

Original languageEnglish (US)
Article number309
Pages (from-to)285-289
Number of pages5
JournalNanotechnology
Volume13
Issue number3
DOIs
StatePublished - Jun 2002

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'An electrochemical detector array to study cell biology on the nanoscale'. Together they form a unique fingerprint.

Cite this