An efficient method for conjugation of thiamine to proteins

M. Jayamani; Philip S. Low

doi:10.1016/S0960-894X(00)80607-1

An efficient method for conjugation of thiamine to proteins

M. Jayamani, Philip S. Low

Research output: Contribution to journal › Article › peer-review

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Abstract

New derivatives of thiamine were prepared by reacting succinic, glutaric or maleic anhydride with the hydroxyl group of thiamine. The resulting free carboxyl group was then activated using N, N, N′, N′-tetramethyl-O-(-N-succinimidyl)uronium tetrafluoroborate¹ 3 and conjugated to proteins. Alternatively, proteins were reacted with 2-iminothiolane and the resulting sulfhydryl groups were then added across the double bond of thiamine monomaleiate 2c prepared by the above method.

Original language	English (US)
Pages (from-to)	1007-1012
Number of pages	6
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	2
Issue number	9
DOIs	https://doi.org/10.1016/S0960-894X(00)80607-1
State	Published - Sep 1992

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/S0960-894X(00)80607-1

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abstract = "New derivatives of thiamine were prepared by reacting succinic, glutaric or maleic anhydride with the hydroxyl group of thiamine. The resulting free carboxyl group was then activated using N, N, N′, N′-tetramethyl-O-(-N-succinimidyl)uronium tetrafluoroborate1 3 and conjugated to proteins. Alternatively, proteins were reacted with 2-iminothiolane and the resulting sulfhydryl groups were then added across the double bond of thiamine monomaleiate 2c prepared by the above method.",

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AU - Low, Philip S.

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AB - New derivatives of thiamine were prepared by reacting succinic, glutaric or maleic anhydride with the hydroxyl group of thiamine. The resulting free carboxyl group was then activated using N, N, N′, N′-tetramethyl-O-(-N-succinimidyl)uronium tetrafluoroborate1 3 and conjugated to proteins. Alternatively, proteins were reacted with 2-iminothiolane and the resulting sulfhydryl groups were then added across the double bond of thiamine monomaleiate 2c prepared by the above method.

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An efficient method for conjugation of thiamine to proteins

Abstract

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