An efficient method for conjugation of thiamine to proteins

M. Jayamani, Philip S. Low

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

New derivatives of thiamine were prepared by reacting succinic, glutaric or maleic anhydride with the hydroxyl group of thiamine. The resulting free carboxyl group was then activated using N, N, N′, N′-tetramethyl-O-(-N-succinimidyl)uronium tetrafluoroborate1 3 and conjugated to proteins. Alternatively, proteins were reacted with 2-iminothiolane and the resulting sulfhydryl groups were then added across the double bond of thiamine monomaleiate 2c prepared by the above method.

Original languageEnglish (US)
Pages (from-to)1007-1012
Number of pages6
JournalBioorganic and Medicinal Chemistry Letters
Volume2
Issue number9
DOIs
StatePublished - Sep 1992

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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