TY - JOUR
T1 - An array of planar apertures for near-field fluorescence correlation spectroscopy
AU - Kelly, Christopher V.
AU - Baird, Barbara A.
AU - Craighead, Harold G.
N1 - Funding Information:
C.V.K. is supported by a National Institutes of Health Kirschstein National Research Service Award (F32-GM092106). Additional research support came from the National Institutes of Health (AI18306) and the Nanobiotechnology Center at Cornell University (NSF ECS-9876771). Device fabrication was performed in the Cornell Nanoscale Science and Technology Facility (NSF ECS-9731293).
PY - 2011/4/6
Y1 - 2011/4/6
N2 - We have developed a method of performing near-field fluorescence correlation spectroscopy via an array of planarized circular apertures of 50 nm diameter. This technique provides 1 μs and 60 nm resolution on proximal samples, including live cells, without incorporating a scanning probe or pulsed lasers or requiring penetration of the sample into the aperture. Millions of apertures are created in an array within a thin film of aluminum on a coverslip and planarized to achieve no height distinction between the apertures and the surrounding metal. Supported lipid bilayers and plasma membranes from live cells adhere to the top of this substrate. We performed fluorescence correlation spectroscopy to demonstrate the sub-diffractionlimited illumination with these devices.
AB - We have developed a method of performing near-field fluorescence correlation spectroscopy via an array of planarized circular apertures of 50 nm diameter. This technique provides 1 μs and 60 nm resolution on proximal samples, including live cells, without incorporating a scanning probe or pulsed lasers or requiring penetration of the sample into the aperture. Millions of apertures are created in an array within a thin film of aluminum on a coverslip and planarized to achieve no height distinction between the apertures and the surrounding metal. Supported lipid bilayers and plasma membranes from live cells adhere to the top of this substrate. We performed fluorescence correlation spectroscopy to demonstrate the sub-diffractionlimited illumination with these devices.
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U2 - 10.1016/j.bpj.2011.02.034
DO - 10.1016/j.bpj.2011.02.034
M3 - Article
C2 - 21463570
AN - SCOPUS:79959651707
VL - 100
SP - L34-L36
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 7
ER -