An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy

D. Xie, W. F. Zhu, H. Cheng, Z. Y. Yao, M. Li, Y. L. Zhao

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A sensitive, and single step reaction assay of glycoproteins in antibody-free mode was proposed on a 4-mercapto-phenylboronic acid (4-MPBA) modified silver nanoparticle film (AgNF) by using surface enhanced Raman scattering (SERS). 4-MPBA/AgNF was designed both as a glycoprotein-specific recognition biosensor and as a Raman reporter for the SERS platform. Variation in the ratio I1574/I1586 for 4-MPBA/AgNF in response to glycoprotein capture constitutes the SERS assay which exhibits high sensitivity and selectivity against nonglycoproteins and is shown to function in complex samples.

Original languageEnglish (US)
Pages (from-to)8881-8886
Number of pages6
JournalPhysical Chemistry Chemical Physics
Volume20
Issue number13
DOIs
StatePublished - 2018

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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