An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy

D. Xie; W. F. Zhu; H. Cheng; Z. Y. Yao; M. Li; Y. L. Zhao

doi:10.1039/c7cp08478a

An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy

D. Xie, W. F. Zhu, H. Cheng, Z. Y. Yao, M. Li, Y. L. Zhao

Houston Methodist

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

A sensitive, and single step reaction assay of glycoproteins in antibody-free mode was proposed on a 4-mercapto-phenylboronic acid (4-MPBA) modified silver nanoparticle film (AgNF) by using surface enhanced Raman scattering (SERS). 4-MPBA/AgNF was designed both as a glycoprotein-specific recognition biosensor and as a Raman reporter for the SERS platform. Variation in the ratio I₁₅₇₄/I₁₅₈₆ for 4-MPBA/AgNF in response to glycoprotein capture constitutes the SERS assay which exhibits high sensitivity and selectivity against nonglycoproteins and is shown to function in complex samples.

Original language	English (US)
Pages (from-to)	8881-8886
Number of pages	6
Journal	Physical Chemistry Chemical Physics
Volume	20
Issue number	13
DOIs	https://doi.org/10.1039/c7cp08478a
State	Published - 2018

Keywords

Chemistry Techniques, Analytical/methods
Glycoproteins/analysis
Spectrum Analysis, Raman

ASJC Scopus subject areas

General Physics and Astronomy
Physical and Theoretical Chemistry

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10.1039/c7cp08478a

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title = "An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy",

abstract = "A sensitive, and single step reaction assay of glycoproteins in antibody-free mode was proposed on a 4-mercapto-phenylboronic acid (4-MPBA) modified silver nanoparticle film (AgNF) by using surface enhanced Raman scattering (SERS). 4-MPBA/AgNF was designed both as a glycoprotein-specific recognition biosensor and as a Raman reporter for the SERS platform. Variation in the ratio I1574/I1586 for 4-MPBA/AgNF in response to glycoprotein capture constitutes the SERS assay which exhibits high sensitivity and selectivity against nonglycoproteins and is shown to function in complex samples.",

keywords = "Chemistry Techniques, Analytical/methods, Glycoproteins/analysis, Spectrum Analysis, Raman",

author = "D. Xie and Zhu, {W. F.} and H. Cheng and Yao, {Z. Y.} and M. Li and Zhao, {Y. L.}",

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year = "2018",

doi = "10.1039/c7cp08478a",

language = "English (US)",

volume = "20",

pages = "8881--8886",

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T1 - An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy

AU - Xie, D.

AU - Zhu, W. F.

AU - Cheng, H.

AU - Yao, Z. Y.

AU - Li, M.

AU - Zhao, Y. L.

PY - 2018

Y1 - 2018

N2 - A sensitive, and single step reaction assay of glycoproteins in antibody-free mode was proposed on a 4-mercapto-phenylboronic acid (4-MPBA) modified silver nanoparticle film (AgNF) by using surface enhanced Raman scattering (SERS). 4-MPBA/AgNF was designed both as a glycoprotein-specific recognition biosensor and as a Raman reporter for the SERS platform. Variation in the ratio I1574/I1586 for 4-MPBA/AgNF in response to glycoprotein capture constitutes the SERS assay which exhibits high sensitivity and selectivity against nonglycoproteins and is shown to function in complex samples.

AB - A sensitive, and single step reaction assay of glycoproteins in antibody-free mode was proposed on a 4-mercapto-phenylboronic acid (4-MPBA) modified silver nanoparticle film (AgNF) by using surface enhanced Raman scattering (SERS). 4-MPBA/AgNF was designed both as a glycoprotein-specific recognition biosensor and as a Raman reporter for the SERS platform. Variation in the ratio I1574/I1586 for 4-MPBA/AgNF in response to glycoprotein capture constitutes the SERS assay which exhibits high sensitivity and selectivity against nonglycoproteins and is shown to function in complex samples.

KW - Chemistry Techniques, Analytical/methods

KW - Glycoproteins/analysis

KW - Spectrum Analysis, Raman

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JO - Physical Chemistry Chemical Physics

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An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy

Abstract

Keywords

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