An analysis of the epidemic of klebsiella pneumoniae carbapenemase-producing k. pneumoniae: Convergence of two evolutionary mechanisms creates the "perfect storm"

Laura J. Rojas, George M. Weinstock, Elsa De La Cadena, Lorena Diaz, Rafael Rios, Blake M. Hanson, Joseph S. Brown, Purva Vats, Daniel S. Phillips, Hoan Nguyen, Kristine M. Hujer, Adriana Correa, Mark D. Adams, Federico Perez, Erica Sodergren, Apurva Narechania, Paul J. Planet, Maria V. Villegas, Rober T.A. Bonomo, Cesar A. Arias

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Background. Carbapenem resistance is a critical healthcare challenge worldwide. Particularly concerning is the widespread dissemination of Klebsiella pneumoniae carbapenemase (KPC). Klebsiella pneumoniae harboring blaKPC (KPC-Kpn) is endemic in many areas including the United States, where the epidemic was primarily mediated by the clonal dissemination of Kpn ST258. We postulated that the spread of blaKPC in other regions occurs by different and more complex mechanisms. To test this, we investigated the evolution and dynamics of spread of KPC-Kpn in Colombia, where KPC became rapidly endemic afer emerging in 2005. Methods. We sequenced the genomes of 133 clinical isolates recovered from 24 tertiary care hospitals located in 10 cities throughout Colombia, between 2002 (before the emergence of KPC-Kpn) and 2014. Phylogenetic reconstructions and evolutionary mapping were performed to determine temporal and genetic associations between the isolates. Results. Our results indicate that the start of the epidemic was driven by horizontal dissemination of mobile genetic elements carrying blaKPC-2, followed by the introduction and subsequent spread of clonal group 258 (CG258) isolates containing blaKPC-3. Conclusions. Te combination of 2 evolutionary mechanisms of KPC-Kpn within a challenged health system of a developing country created the "perfect storm" for sustained endemicity of these multidrug-resistant organisms in Colombia.

Original languageEnglish (US)
Pages (from-to)82-92
Number of pages11
JournalJournal of Infectious Diseases
Volume217
Issue number1
DOIs
StatePublished - Jan 1 2018

Keywords

  • Colombia
  • KPC
  • Klebsiella pneumoniae

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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