Amylase in urine as measured by a single-step chromolytic method

R. L. Bertholf, E. S. Winn-Deen, D. E. Bruns

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We studied a new single-step direct chromolytic method (Behring D.A.T.) for measuring urinary amylase (1,4-α-D-glucan glucanohydrolase, EC 3.2.1.1) activity, comparing results with those by a similar, but two-step, procedure that requires an auxiliary (coupling) enzyme. The two methods gave virtually identical relative responses to purified human pancreatic and salivary amylases. Assay of four quality-control materials to evaluate the total (day-to-day) precision of the new method yielded CVs of 4 to 7%, similar to those of the comparison method for each of the four quality-control samples. Amylase activity was measured by both methods in 110 random (i.e., untimed) urine specimens. Linear regression analysis provided a slope and y-intercept of 0.947 and 4 U/L (x = comparison method, y = direct method), respectively, and a standard error of the estimate of 25 U/L for specimens in which the amylase activities ranged from 11 to 1465 U/L (mean = 358 U/L) by the comparison method. The mean rate of amylase excretion in 2-h timed urine specimens from 95 healthy volunteers, as measured by the new method, was 7.18 (SD = 3.18) U/h, and the nonparametric (95% confidence interval) reference interval was 1.6 to 15.2 U/h. We consider the new method a promising alternative to other kinetic assays that require the use of auxiliary enzymes.

Original languageEnglish (US)
Pages (from-to)754-757
Number of pages4
JournalClinical Chemistry
Volume34
Issue number4
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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