Amplification and expression of the c‐erb B‐2/neu proto‐oncogene in human bladder cancer

Haiyen E. Zhau, Xin Zhang, Andrew C. Von Eschenbach, Kathleen Scorsone, R. Joseph Babain, Jae Y. Ro, Mien‐Chie ‐C Hung

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113 Scopus citations


The c-erb B-2/neu gene encodes a cell-surface glycoprotein with extensive homology to, but distinct from, the epidermal growth-factor receptor. In this study, we compared the c-erb B-2/neu gene amplification and expression of tissue specimens obtained from the bladders of normal controls and patients with high-grade transitional cell bladder carcinoma. Southern blot analysis of DNAs from 24 patients and 5 controls showed 2 cases of c-erb B-2/neu gene amplification in patients and none in controls. Western blot analysis demonstrated that c-erb B-2/neu was expressed in 67.6% (23/34) of patient specimens but in none of the controls (0/5). This finding agreed with the result of immunohistochemical staining, which showed that tissue from 74.3% (26/35) of the patients and none of the controls (0/7) showed positive immunofluorescence staining. This is the first report suggesting that c-erb B-2/neu gene amplification and expression may be associated with human bladder carcinogenesis.

Original languageEnglish (US)
Pages (from-to)254-257
Number of pages4
JournalMolecular Carcinogenesis
Issue number5
StatePublished - 1990


  • bladder carcinogenesis
  • Neu gene
  • tumor marker

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology


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