Amperometric glucose biosensor based on electroconductive hydrogels

Christian N. Kotanen, Chaker Tlili, Anthony Guiseppi-Elie

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Fabrication of an enzyme amperometric biosensor for glucose via electropolymerization of pyrrole in the presence of glucose oxidase onto a hydrogel coated platinum electrode is hereby established as a viable biotransducer fabrication method. Platinum micro- (φ=25 mm) and macro- (φ=100 μm) electrodes were electrochemically activated and chemically modified with 3-aminopropyl-trimethoxysilane (APTMS), functionalized with acryloyl(polyethyleneglycol)-N-hydroxysuccinamide (ACRL-PEG-NHS), dipped into a polyHEMA based hydrogel cocktail and UV cross-linked. Electropolymerization of Py in the presence of GOx produced glucose responsive biotransducers that showed; (i) a 4-fold reduction in sensitivity compared with directly electropolymerized PPy films, (ii) an electropolymerization charge density dependence of biotransducer sensitivity and enzyme activity that was maximal at 1.0 mC/cm2 with an apparent KM of 33 mM, (iii) interference screening of ascorbic acid and (iv) a temporal increase in sensitivity with storage over a 17 days period. This method has the ability to precisely and quantitatively add enzyme catalytic bioactivity to metal or semiconductor biointerfaces for applications in biosensors, bioelectronics and bionics.

Original languageEnglish (US)
Pages (from-to)228-235
Number of pages8
JournalTalanta
Volume103
DOIs
StatePublished - Jan 15 2013

Keywords

  • Amperometry
  • Biofabrication
  • Biosensors
  • Biotransducers
  • Glucose
  • Glucose oxidase
  • Hydrogels
  • Membranes

ASJC Scopus subject areas

  • Analytical Chemistry

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