Amino-functionalized CdSe/ZnS quantum dot-based lateral flow immunoassay for sensitive detection of aflatoxin B1

Research output: Contribution to journalArticle

Jieying Li, Mao Mao, Feng Wu, Qiang Li, Luyao Wei, Lan Ma

In this study, highly fluorescent amino-functionalized CdSe/ZnS core/shell quantum dots (CdSe/ZnS QDs) coated with amphiphilic N-alkylated poly(ethyleneimine) have been synthesized and applied as fluorescent probes in lateral flow immunoassay (LFIA) strips for on-site determination of aflatoxin B1 (AFB1). The amino-functionalized QDs were used to combine with anti-AFB1 via thiol-maleimide conjugation and they effectively enhanced the detection sensitivity. The test strip had high sensitivity for AFB1 from 0.001 ng mL-1 to 10 ng mL-1 with a low limit of detection (LOD) of 0.005 ng mL-1, which was much lower than the allowable concentration by the European Commission (EC) (2.0 μg kg-1) and FDA (20 μg kg-1). The IC50 value was calculated to be 0.025 ng mL-1. The reproducibility and recovery tests showed that the quantitative results were accurate, and it could be used for rapid on-site detection of AFB1 in cereal products.

Original languageEnglish (US)
Pages (from-to)3582-3588
Number of pages7
JournalAnalytical Methods
Volume10
Issue number29
DOIs
StatePublished - Aug 7 2018

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Amino-functionalized CdSe/ZnS quantum dot-based lateral flow immunoassay for sensitive detection of aflatoxin B1. / Li, Jieying; Mao, Mao; Wu, Feng; Li, Qiang; Wei, Luyao; Ma, Lan.

In: Analytical Methods, Vol. 10, No. 29, 07.08.2018, p. 3582-3588.

Research output: Contribution to journalArticle

Harvard

Li, J, Mao, M, Wu, F, Li, Q, Wei, L & Ma, L 2018, 'Amino-functionalized CdSe/ZnS quantum dot-based lateral flow immunoassay for sensitive detection of aflatoxin B1' Analytical Methods, vol. 10, no. 29, pp. 3582-3588. DOI: 10.1039/c8ay00608c

APA

Li, J., Mao, M., Wu, F., Li, Q., Wei, L., & Ma, L. (2018). Amino-functionalized CdSe/ZnS quantum dot-based lateral flow immunoassay for sensitive detection of aflatoxin B1. Analytical Methods, 10(29), 3582-3588. DOI: 10.1039/c8ay00608c

Vancouver

Li J, Mao M, Wu F, Li Q, Wei L, Ma L. Amino-functionalized CdSe/ZnS quantum dot-based lateral flow immunoassay for sensitive detection of aflatoxin B1. Analytical Methods. 2018 Aug 7;10(29):3582-3588. Available from, DOI: 10.1039/c8ay00608c

Author

Li, Jieying ; Mao, Mao ; Wu, Feng ; Li, Qiang ; Wei, Luyao ; Ma, Lan. / Amino-functionalized CdSe/ZnS quantum dot-based lateral flow immunoassay for sensitive detection of aflatoxin B1. In: Analytical Methods. 2018 ; Vol. 10, No. 29. pp. 3582-3588

BibTeX

@article{c7ce6e562f11441b98f017d19b033c0b,
title = "Amino-functionalized CdSe/ZnS quantum dot-based lateral flow immunoassay for sensitive detection of aflatoxin B1",
abstract = "In this study, highly fluorescent amino-functionalized CdSe/ZnS core/shell quantum dots (CdSe/ZnS QDs) coated with amphiphilic N-alkylated poly(ethyleneimine) have been synthesized and applied as fluorescent probes in lateral flow immunoassay (LFIA) strips for on-site determination of aflatoxin B1 (AFB1). The amino-functionalized QDs were used to combine with anti-AFB1 via thiol-maleimide conjugation and they effectively enhanced the detection sensitivity. The test strip had high sensitivity for AFB1 from 0.001 ng mL-1 to 10 ng mL-1 with a low limit of detection (LOD) of 0.005 ng mL-1, which was much lower than the allowable concentration by the European Commission (EC) (2.0 μg kg-1) and FDA (20 μg kg-1). The IC50 value was calculated to be 0.025 ng mL-1. The reproducibility and recovery tests showed that the quantitative results were accurate, and it could be used for rapid on-site detection of AFB1 in cereal products.",
author = "Jieying Li and Mao Mao and Feng Wu and Qiang Li and Luyao Wei and Lan Ma",
year = "2018",
month = "8",
day = "7",
doi = "10.1039/c8ay00608c",
language = "English (US)",
volume = "10",
pages = "3582--3588",
journal = "Analytical Methods",
issn = "1759-9660",
publisher = "Royal Society of Chemistry",
number = "29",

}

RIS

TY - JOUR

T1 - Amino-functionalized CdSe/ZnS quantum dot-based lateral flow immunoassay for sensitive detection of aflatoxin B1

AU - Li,Jieying

AU - Mao,Mao

AU - Wu,Feng

AU - Li,Qiang

AU - Wei,Luyao

AU - Ma,Lan

PY - 2018/8/7

Y1 - 2018/8/7

N2 - In this study, highly fluorescent amino-functionalized CdSe/ZnS core/shell quantum dots (CdSe/ZnS QDs) coated with amphiphilic N-alkylated poly(ethyleneimine) have been synthesized and applied as fluorescent probes in lateral flow immunoassay (LFIA) strips for on-site determination of aflatoxin B1 (AFB1). The amino-functionalized QDs were used to combine with anti-AFB1 via thiol-maleimide conjugation and they effectively enhanced the detection sensitivity. The test strip had high sensitivity for AFB1 from 0.001 ng mL-1 to 10 ng mL-1 with a low limit of detection (LOD) of 0.005 ng mL-1, which was much lower than the allowable concentration by the European Commission (EC) (2.0 μg kg-1) and FDA (20 μg kg-1). The IC50 value was calculated to be 0.025 ng mL-1. The reproducibility and recovery tests showed that the quantitative results were accurate, and it could be used for rapid on-site detection of AFB1 in cereal products.

AB - In this study, highly fluorescent amino-functionalized CdSe/ZnS core/shell quantum dots (CdSe/ZnS QDs) coated with amphiphilic N-alkylated poly(ethyleneimine) have been synthesized and applied as fluorescent probes in lateral flow immunoassay (LFIA) strips for on-site determination of aflatoxin B1 (AFB1). The amino-functionalized QDs were used to combine with anti-AFB1 via thiol-maleimide conjugation and they effectively enhanced the detection sensitivity. The test strip had high sensitivity for AFB1 from 0.001 ng mL-1 to 10 ng mL-1 with a low limit of detection (LOD) of 0.005 ng mL-1, which was much lower than the allowable concentration by the European Commission (EC) (2.0 μg kg-1) and FDA (20 μg kg-1). The IC50 value was calculated to be 0.025 ng mL-1. The reproducibility and recovery tests showed that the quantitative results were accurate, and it could be used for rapid on-site detection of AFB1 in cereal products.

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U2 - 10.1039/c8ay00608c

DO - 10.1039/c8ay00608c

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SP - 3582

EP - 3588

JO - Analytical Methods

T2 - Analytical Methods

JF - Analytical Methods

SN - 1759-9660

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ER -

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