Amino-functionalized CdSe/ZnS quantum dot-based lateral flow immunoassay for sensitive detection of aflatoxin B1

Jieying Li, Mao Mao, Feng Wu, Qiang Li, Luyao Wei, Lan Ma

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

In this study, highly fluorescent amino-functionalized CdSe/ZnS core/shell quantum dots (CdSe/ZnS QDs) coated with amphiphilic N-alkylated poly(ethyleneimine) have been synthesized and applied as fluorescent probes in lateral flow immunoassay (LFIA) strips for on-site determination of aflatoxin B1 (AFB1). The amino-functionalized QDs were used to combine with anti-AFB1 via thiol-maleimide conjugation and they effectively enhanced the detection sensitivity. The test strip had high sensitivity for AFB1 from 0.001 ng mL-1 to 10 ng mL-1 with a low limit of detection (LOD) of 0.005 ng mL-1, which was much lower than the allowable concentration by the European Commission (EC) (2.0 μg kg-1) and FDA (20 μg kg-1). The IC50 value was calculated to be 0.025 ng mL-1. The reproducibility and recovery tests showed that the quantitative results were accurate, and it could be used for rapid on-site detection of AFB1 in cereal products.

Original languageEnglish (US)
Pages (from-to)3582-3588
Number of pages7
JournalAnalytical Methods
Volume10
Issue number29
DOIs
StatePublished - Aug 7 2018

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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