Amiloride-sensitive channels in marginal cells in the stria vascularis of the guinea pig cochlea

K. H. Iwasa, K. Mizuta, David J. Lim, D. J. Benos, M. Tachibana

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We examined marginal cells in stria vascularis for the presence of amiloride-sensitive Na+ channels, a possible pathway for maintaining a low Na+ concentration in the endolymph. Whole-cell voltage-clamp experiment shows that amiloride at 1 μM concentration reversibly reduces inward current more than outward current. Immunogold-labeling method shows that the luminal and lateral membrane have antigenic sites for these antibodies. These observations indicate the presence of amiloride-sensitive channels in the marginal cell. If amiloride-sensitive channels in the luminal membrane are highly selective to Na+, they could be an efficient pathway for Na+ uptake from the endolymph. In the basolateral membrane, amiloride-sensitive Na+ channels may make a relatively small contribution to the unusual resting potential.

Original languageEnglish (US)
Pages (from-to)163-166
Number of pages4
JournalNeuroscience Letters
Volume172
Issue number1-2
DOIs
StatePublished - May 19 1994

Keywords

  • Amiloride
  • Endolymph
  • Sodium channel
  • Stria vascularis

ASJC Scopus subject areas

  • Neuroscience(all)

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