Amelioration of the physiologic and biochemical changes of acute pancreatitis using an anti-TNF-α polyclonal antibody

Hani P. Grewal, Aboubaker Mohey el Din, Lillian Gaber, Malak Kotb, A. Osama Gaber

Research output: Contribution to journalArticle

186 Scopus citations

Abstract

Tumor necrosis factor (TNF) is an inflammatory cytokine that may be an important mediator in the development of the systemic sequelae associated with severe acute pancreatitis. The purpose of this study was to determine whether the neutralization of TNF-α with a polyclonal antibody could ameliorate selected biochemical parameters of severe pancreatitis in a rat model. Pancreatitis was induced by an antegrade injection of artificial bile into the bile duct. Forty rats were randomized into 4 groups: no surgery (controls), saline infusion to bile duct (sham), placebo treatment in animals with pancreatitis (placebo + Px), and pretreatment with a polyclonal antibody (PAb) in animals with pancreatitis (PAb + Px). Serum TNF-α, amylase, calcium, hematocrit, glucose, and ascites volume were measured 2 hours after bile duct infusion. Pretreatment with the PAb produced a significant improvement in all parameters when compared with pancreatitis animals treated with placebo (p < 0.001). In addition, TNF-α, which was elevated in animals with pancreatitis, was reduced significantly in treated animals (p < 0.001). These results suggest that TNF-α may be an important mediator in the evolution of the systemic manifestations of severe acute pancreatitis.

Original languageEnglish (US)
Pages (from-to)214-219
Number of pages6
JournalThe American Journal of Surgery
Volume167
Issue number1
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Surgery

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