TY - JOUR
T1 - Altered expression of retinoblastoma protein and known prognostic variables in locally advanced bladder cancer
AU - Logothetis, Christopher J.
AU - Xu, Hong Ji
AU - Ro, Jae
AU - Hu, Shi Xue
AU - Sahin, Aysegul
AU - Ordonez, Nelson
AU - Benedict, William F.
N1 - Funding Information:
Received April 13, 1992; revised May 29, 1992; accepted June 5, 1992. Supported by Public Health Service grants CA-54672 and CA-16672 from the National Cancer Institute, National Institutes of Health, Department of Health and Human Services; and by Texas Advanced Technology Program grant 4949019. C. J. Logothetis (Department of Medical Oncology), J. Y. Ro, A. Sahin, N. Ordonez (Department of Pathology), The University of Texas M. D. Anderson Cancer Center. Houston. H.-J. Xu, S.-X. Hu, W. F. Benedict, Center for Biotechnology, Baylor College of Medicine, The Woodlands, Tex. *Correspondence to: Christopher J. Logothetis, M.D., Department of Medical Oncology, Box 13, The University of Texas M. D. Anderson Cancer Center. 1515 Holcombe Blvd., Houston, TX 77030.
PY - 1992/8/19
Y1 - 1992/8/19
N2 - Background: The clinical behavior of the tumor in patients with locally advanced bladder carcinoma is unpredictable. Current predictors of clinical behavior include depth of muscle invasion, presence of vascular invasion, proliferation rate, and loss of blood group antigens. Treatment selection would be facilitated by the development of a reliable marker of tumor progression. Functional retinoblastoma (RB) gene loss has been reported to occur in bladder carcinoma, but the significance of this loss is unknown. Purpose: We have evaluated the frequency of functional loss of the RB gene in locally advanced bladder carcinoma and have compared the results to known prognostic factors in the same cohort. Methods: Forty-three study patients with pathologically well-characterized, locally advanced bladder carcinoma, who were placed in a protocol incorporating surgery and chemotherapy, were studied for known clinical and pathological prognostic indicators as well as for their Rb status. Formalin-fixed and paraffin-embedded archival primary tumor tissues were used for histological and immunohistochemical analyses. Results: Altered Rb protein expression was documented in 37% of the tumor specimens. The high rate of altered Rb expression found in this cohort with advanced urothelial tumors strongly suggests that RB functional loss may be associated with tumor progression in this malignancy. Altered Rb protein expression was found to be independent of other known prognostic variables. A significantly poorer tumor-free survival rate also was noted for those patients who had a tumor with an altered Rb protein with or without vascular invasion. Conclusion: The high frequency of Rb alteration in locally advanced bladder carcinomas, plus the fact that a significant correlation could not be found between the Rb status and other known prognostic markers in this preliminary study, suggests that altered RB expression may be an independent prognostic marker of tumor progression in bladder cancer.
AB - Background: The clinical behavior of the tumor in patients with locally advanced bladder carcinoma is unpredictable. Current predictors of clinical behavior include depth of muscle invasion, presence of vascular invasion, proliferation rate, and loss of blood group antigens. Treatment selection would be facilitated by the development of a reliable marker of tumor progression. Functional retinoblastoma (RB) gene loss has been reported to occur in bladder carcinoma, but the significance of this loss is unknown. Purpose: We have evaluated the frequency of functional loss of the RB gene in locally advanced bladder carcinoma and have compared the results to known prognostic factors in the same cohort. Methods: Forty-three study patients with pathologically well-characterized, locally advanced bladder carcinoma, who were placed in a protocol incorporating surgery and chemotherapy, were studied for known clinical and pathological prognostic indicators as well as for their Rb status. Formalin-fixed and paraffin-embedded archival primary tumor tissues were used for histological and immunohistochemical analyses. Results: Altered Rb protein expression was documented in 37% of the tumor specimens. The high rate of altered Rb expression found in this cohort with advanced urothelial tumors strongly suggests that RB functional loss may be associated with tumor progression in this malignancy. Altered Rb protein expression was found to be independent of other known prognostic variables. A significantly poorer tumor-free survival rate also was noted for those patients who had a tumor with an altered Rb protein with or without vascular invasion. Conclusion: The high frequency of Rb alteration in locally advanced bladder carcinomas, plus the fact that a significant correlation could not be found between the Rb status and other known prognostic markers in this preliminary study, suggests that altered RB expression may be an independent prognostic marker of tumor progression in bladder cancer.
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U2 - 10.1093/jnci/84.16.1256
DO - 10.1093/jnci/84.16.1256
M3 - Article
C2 - 1640485
AN - SCOPUS:0026673062
SN - 0027-8874
VL - 84
SP - 1256
EP - 1261
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 16
ER -