Alpha-1 subunits of voltage gated Ca2+ channels in the mesencephalon × neuroblastoma hybrid cell line MES23.5

T. Schneider; E. Perez-Reyes; O. Nyormoi; X. Wei; G. D. Crawford; R. G. Smith; Stanley H. Appel; L. Birnbaumer

doi:10.1016/0306-4522(95)00147-B

Alpha-1 subunits of voltage gated Ca²⁺ channels in the mesencephalon × neuroblastoma hybrid cell line MES23.5

T. Schneider, E. Perez-Reyes, O. Nyormoi, X. Wei, G. D. Crawford, R. G. Smith, Stanley H. Appel, L. Birnbaumer

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The identity of α₁ subunits from voltage operated Ca²⁺ channels was determined in the rat/mouse mesencephalon × N18TG2 hybridoma cell line MES23.5, by sequence analysis of reverse transcription-polymerase chain reaction products and antagonist binding. Sequences were derived from the L-(α_1D, Q-(α_A) and ω-contoxin GVIA sensitive N-type (α_1B) Ca²⁺ channel α₁ subunits. The amplified fragments, which are homologous to the region between domain III and IV of known α₁ subunits, reveal splice variation in the L- and Q-type α₁ subunit of MES23.5 cells. The transcripts of α₁ subunits in these cells were quantified by RNAase protection assay. The data show the existence of different Ca²⁺ channel types in a single cell line and may reflect multiple functions of voltage operated Ca²⁺ channels during growth, differentiation and transmitter release.

Original language	English (US)
Pages (from-to)	479-485
Number of pages	7
Journal	Neuroscience
Volume	68
Issue number	2
DOIs	https://doi.org/10.1016/0306-4522(95)00147-B
State	Published - Sep 1995

ASJC Scopus subject areas

General Neuroscience

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@article{3390264edc9e4401ba47c91dec07619b,

title = "Alpha-1 subunits of voltage gated Ca2+ channels in the mesencephalon × neuroblastoma hybrid cell line MES23.5",

abstract = "The identity of α1 subunits from voltage operated Ca2+ channels was determined in the rat/mouse mesencephalon × N18TG2 hybridoma cell line MES23.5, by sequence analysis of reverse transcription-polymerase chain reaction products and antagonist binding. Sequences were derived from the L-(α1D, Q-(αA) and ω-contoxin GVIA sensitive N-type (α1B) Ca2+ channel α1 subunits. The amplified fragments, which are homologous to the region between domain III and IV of known α1 subunits, reveal splice variation in the L- and Q-type α1 subunit of MES23.5 cells. The transcripts of α1 subunits in these cells were quantified by RNAase protection assay. The data show the existence of different Ca2+ channel types in a single cell line and may reflect multiple functions of voltage operated Ca2+ channels during growth, differentiation and transmitter release.",

author = "T. Schneider and E. Perez-Reyes and O. Nyormoi and X. Wei and Crawford, \{G. D.\} and Smith, \{R. G.\} and Appel, \{Stanley H.\} and L. Birnbaumer",

year = "1995",

month = sep,

doi = "10.1016/0306-4522(95)00147-B",

language = "English (US)",

volume = "68",

pages = "479--485",

journal = "Neuroscience",

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T1 - Alpha-1 subunits of voltage gated Ca2+ channels in the mesencephalon × neuroblastoma hybrid cell line MES23.5

AU - Schneider, T.

AU - Perez-Reyes, E.

AU - Nyormoi, O.

AU - Wei, X.

AU - Crawford, G. D.

AU - Smith, R. G.

AU - Appel, Stanley H.

AU - Birnbaumer, L.

PY - 1995/9

Y1 - 1995/9

N2 - The identity of α1 subunits from voltage operated Ca2+ channels was determined in the rat/mouse mesencephalon × N18TG2 hybridoma cell line MES23.5, by sequence analysis of reverse transcription-polymerase chain reaction products and antagonist binding. Sequences were derived from the L-(α1D, Q-(αA) and ω-contoxin GVIA sensitive N-type (α1B) Ca2+ channel α1 subunits. The amplified fragments, which are homologous to the region between domain III and IV of known α1 subunits, reveal splice variation in the L- and Q-type α1 subunit of MES23.5 cells. The transcripts of α1 subunits in these cells were quantified by RNAase protection assay. The data show the existence of different Ca2+ channel types in a single cell line and may reflect multiple functions of voltage operated Ca2+ channels during growth, differentiation and transmitter release.

AB - The identity of α1 subunits from voltage operated Ca2+ channels was determined in the rat/mouse mesencephalon × N18TG2 hybridoma cell line MES23.5, by sequence analysis of reverse transcription-polymerase chain reaction products and antagonist binding. Sequences were derived from the L-(α1D, Q-(αA) and ω-contoxin GVIA sensitive N-type (α1B) Ca2+ channel α1 subunits. The amplified fragments, which are homologous to the region between domain III and IV of known α1 subunits, reveal splice variation in the L- and Q-type α1 subunit of MES23.5 cells. The transcripts of α1 subunits in these cells were quantified by RNAase protection assay. The data show the existence of different Ca2+ channel types in a single cell line and may reflect multiple functions of voltage operated Ca2+ channels during growth, differentiation and transmitter release.

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U2 - 10.1016/0306-4522(95)00147-B

DO - 10.1016/0306-4522(95)00147-B

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SN - 0306-4522

VL - 68

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JO - Neuroscience

JF - Neuroscience

IS - 2

ER -

Alpha-1 subunits of voltage gated Ca²⁺ channels in the mesencephalon × neuroblastoma hybrid cell line MES23.5

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ASJC Scopus subject areas

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