Alpha-1 subunits of voltage gated Ca2+ channels in the mesencephalon × neuroblastoma hybrid cell line MES23.5

T. Schneider, E. Perez-Reyes, O. Nyormoi, X. Wei, G. D. Crawford, R. G. Smith, Stanley H. Appel, L. Birnbaumer

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The identity of α1 subunits from voltage operated Ca2+ channels was determined in the rat/mouse mesencephalon × N18TG2 hybridoma cell line MES23.5, by sequence analysis of reverse transcription-polymerase chain reaction products and antagonist binding. Sequences were derived from the L-(α1D, Q-(αA) and ω-contoxin GVIA sensitive N-type (α1B) Ca2+ channel α1 subunits. The amplified fragments, which are homologous to the region between domain III and IV of known α1 subunits, reveal splice variation in the L- and Q-type α1 subunit of MES23.5 cells. The transcripts of α1 subunits in these cells were quantified by RNAase protection assay. The data show the existence of different Ca2+ channel types in a single cell line and may reflect multiple functions of voltage operated Ca2+ channels during growth, differentiation and transmitter release.

Original languageEnglish (US)
Pages (from-to)479-485
Number of pages7
JournalNeuroscience
Volume68
Issue number2
DOIs
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • Neuroscience(all)

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