Advanced approach to simultaneous monitoring of multiple bacteria in space

Mitchell D. Eggers, William J. Balch, Leopoldo G. Mendoza, Ramprasad Gangadharan, Arnab K. Mallik, Michael G. McMahon, Michael E. Hogan, Du Xaio, Thomas R. Powdrill, Bonnie Iverson, George E. Fox, Richard C. Willson, Karine I. Maillard, Janet L. Siefert, Narinder Singh

Research output: Contribution to journalConference articlepeer-review


A novel microarray-based approach to simultaneous detection, quantitation, and identification of multiple microorganisms found in water and air supplies is presented. The rapid DNA/RNA probe-based approach avoids time consuming cell cultivation procedures by utilizing the inherent amplification property of rRNA combined with highly sensitive proximal CCD detection. Specifically, optimal DNA probes for simultaneously capturing Escherichia coli and Vibrio proteolyticus were arrayed in a 4 x 4 matrix using a novel microarray printer capable of depositing hundreds of probes within a single well (1cm2) of a conventional 96 well microtiter plate. Following hybridization of a 16S rRNA microorganism mixture to the DNA microarray, detection, imaging, quantitation and identification was performed within seconds employing the complimentary PC-based proximal CCD detector/imager.

Original languageEnglish (US)
JournalSAE Technical Papers
StatePublished - 1997
Event27th International Conference on Environmental Systems - Lake Tahoe, NV, United States
Duration: Jul 14 1997Jul 17 1997

ASJC Scopus subject areas

  • Automotive Engineering
  • Safety, Risk, Reliability and Quality
  • Pollution
  • Industrial and Manufacturing Engineering


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