Adult neural stem cells

Rossella Galli, Angela Gritti, Angelo Vescovi

Research output: Chapter in Book/Report/Conference proceedingChapter

19 Scopus citations

Abstract

Neural stem cells (NSCs) have been identified in the mature central nervous system (CNS), and they reside in specific areas. Cultures of NSCs can be successfully established in vitro by exploiting the NeuroSphere assay. This methodology relies on the continuous exposure of neural cells to mitogens such as epidermal growth factor and fibroblast growth factor-2. Under these conditions, only NSCs and highly undifferentiated progenitors proliferate, whereas committed precursors and terminally differentiated cells are eliminated from the culture. The proper application of this method to the cells allows the establishment of long-term expanding stable NSC lines, starting from different neural tissues as the adult rodent CNS and human brain tumor specimens.

Original languageEnglish (US)
Title of host publicationNeural Stem Cells
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages67-84
Number of pages18
ISBN (Print)9781588298461
DOIs
StatePublished - Jan 1 2008

Publication series

NameMethods in Molecular Biology
Volume438
ISSN (Print)1064-3745

Keywords

  • Adult neurogenesis
  • Brain tumor
  • Cancer stem cell
  • Epidermal growth factor
  • Fibroblast growth factor
  • Neural stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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