TY - JOUR
T1 - Adipose-Derived Biogenic Nanoparticles for Suppression of Inflammation
AU - Tian, Ming
AU - Ticer, Taylor
AU - Wang, Qikun
AU - Walker, Sierra
AU - Pham, Anthony
AU - Suh, Annie
AU - Busatto, Sara
AU - Davidovich, Irina
AU - Al-Kharboosh, Rawan
AU - Lewis-Tuffin, Laura
AU - Ji, Baoan
AU - Quinones-Hinojosa, Alfredo
AU - Talmon, Yeshayahu
AU - Shapiro, Shane
AU - Rückert, Felix
AU - Wolfram, Joy
N1 - Funding Information:
This work was supported by Mayo Clinic. Among various sources of intramural funding, the authors particularly acknowledge support from the Center for Regenerative Medicine (JW, SS), Mayo Clinic Florida Focused Research Team Program (JW, SS), the Mayo Clinic Investigator Award (AQH), and the Mayo Professorship Award (AQH). This work was also partially supported by the China Scholarship Council (MT, QKW), Müller-Stiftung Mannheim and Vetter-Stiftung Mannheim (FR), the State of Florida Cancer Award (AQH), Jack & Peggy Sturm Foundation (SS), the United States Department of Defense (DOD) under award number W81XWH-15-1-0257 (BJ), and the United States National Cancer Institute under award numbers R01CA183827 (AQH) and R01CA195503 (AQH). The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies. The cryo-TEM work was performed at the Technion Center for Electron Microscopy of Soft Matter, supported by the Technion Russell Berrie Nanotechnology Institute (RBNI).
Publisher Copyright:
© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Extracellular vesicles secreted from adipose-derived mesenchymal stem cells (ADSCs) have therapeutic effects in inflammatory diseases. However, production of extracellular vesicles (EVs) from ADSCs is costly, inefficient, and time consuming. The anti-inflammatory properties of adipose tissue-derived EVs and other biogenic nanoparticles have not been explored. In this study, biogenic nanoparticles are obtained directly from lipoaspirate, an easily accessible and abundant source of biological material. Compared to ADSC-EVs, lipoaspirate nanoparticles (Lipo-NPs) take less time to process (hours compared to months) and cost less to produce (clinical-grade cell culture facilities are not required). The physicochemical characteristics and anti-inflammatory properties of Lipo-NPs are evaluated and compared to those of patient-matched ADSC-EVs. Moreover, guanabenz loading in Lipo-NPs is evaluated for enhanced anti-inflammatory effects. Apolipoprotein E and glycerolipids are enriched in Lipo-NPs compared to ADSC-EVs. Additionally, the uptake of Lipo-NPs in hepatocytes and macrophages is higher. Lipo-NPs and ADSC-EVs have comparable protective and anti-inflammatory effects. Specifically, Lipo-NPs reduce toll-like receptor 4-induced secretion of inflammatory cytokines in macrophages. Guanabenz-loaded Lipo-NPs further suppress inflammatory pathways, suggesting that this combination therapy can have promising applications for inflammatory diseases.
AB - Extracellular vesicles secreted from adipose-derived mesenchymal stem cells (ADSCs) have therapeutic effects in inflammatory diseases. However, production of extracellular vesicles (EVs) from ADSCs is costly, inefficient, and time consuming. The anti-inflammatory properties of adipose tissue-derived EVs and other biogenic nanoparticles have not been explored. In this study, biogenic nanoparticles are obtained directly from lipoaspirate, an easily accessible and abundant source of biological material. Compared to ADSC-EVs, lipoaspirate nanoparticles (Lipo-NPs) take less time to process (hours compared to months) and cost less to produce (clinical-grade cell culture facilities are not required). The physicochemical characteristics and anti-inflammatory properties of Lipo-NPs are evaluated and compared to those of patient-matched ADSC-EVs. Moreover, guanabenz loading in Lipo-NPs is evaluated for enhanced anti-inflammatory effects. Apolipoprotein E and glycerolipids are enriched in Lipo-NPs compared to ADSC-EVs. Additionally, the uptake of Lipo-NPs in hepatocytes and macrophages is higher. Lipo-NPs and ADSC-EVs have comparable protective and anti-inflammatory effects. Specifically, Lipo-NPs reduce toll-like receptor 4-induced secretion of inflammatory cytokines in macrophages. Guanabenz-loaded Lipo-NPs further suppress inflammatory pathways, suggesting that this combination therapy can have promising applications for inflammatory diseases.
KW - anti-inflammation
KW - exosome
KW - extracellular vesicles
KW - inflammation
KW - lipoaspirate
KW - microvesicle
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U2 - 10.1002/smll.201904064
DO - 10.1002/smll.201904064
M3 - Article
C2 - 32067382
AN - SCOPUS:85079702954
SN - 1613-6810
VL - 16
SP - e1904064
JO - Small
JF - Small
IS - 10
M1 - 1904064
ER -