TY - JOUR
T1 - Adhesion of rat hepatocytes to collagen
AU - Rubin, Kristofer
AU - Oldberg, Åke
AU - Höök, Magnus
AU - Öbrink, Björn
N1 - Funding Information:
Expert technical assistanceb y MS Y. Ekbom, MS I. Petterssona nd MS C. Pihl, is gratefullya cknowledged. We thank Dr A. Vaheri for performing the radio-immuno assav. This invest&rationw as sunuortedb v grants from ihe Swedish M>dical Research Council (nroiect nos. 03X-4, 05200,0 5197,0 2309),G ustaf V:s 8O&sfond, Svenska liv~rslikringsbolags nlmnd for medicinskf orskning,a nd the Medical Faculty, University of Uppsala, Sweden.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1978/11
Y1 - 1978/11
N2 - Rat hepatocytes, freshly isolated with a collagenase perfusion technique, were found to attach within 1 h on collagen substrates and on culture dishes coated with cold insoluble globulin (CIG) or asialoceruloplasmin (AC). Spreading was observed on collagen and CIG but not on AC. Both attachment and spreading occurred in a simple balanced salt solution in the absence of serum. In the absence of serum no attachment was observed on plain plastic dishes or on dishes coated with serum albumin or other plasma proteins, unless divalent manganese ions were present. In the presence of manganese the hepatocytes attached to all surfaces tested, but no spreading occurred. Attachment to collagen occurred equally well to collagens type I or type III both in the native, fibrillar state and in the denatured state. Collagen attachment required magnesium ions but did not appear to involve the collagen-linked carbohydrates. Different mechanisms were found to operate in hepatocyte attachment to collagen and to AC; the latter is most likely mediated by the hepatocyte surface receptor involved in recognition and uptake of asialoglycoproteins. The role of CIG in hepatocyte attachment to collagen was investigated. Data are presented suggesting that this glycoprotein, which mediates the adhesion of fibroblasts to collagen, is not required for hepatocyte attachment to collagen.
AB - Rat hepatocytes, freshly isolated with a collagenase perfusion technique, were found to attach within 1 h on collagen substrates and on culture dishes coated with cold insoluble globulin (CIG) or asialoceruloplasmin (AC). Spreading was observed on collagen and CIG but not on AC. Both attachment and spreading occurred in a simple balanced salt solution in the absence of serum. In the absence of serum no attachment was observed on plain plastic dishes or on dishes coated with serum albumin or other plasma proteins, unless divalent manganese ions were present. In the presence of manganese the hepatocytes attached to all surfaces tested, but no spreading occurred. Attachment to collagen occurred equally well to collagens type I or type III both in the native, fibrillar state and in the denatured state. Collagen attachment required magnesium ions but did not appear to involve the collagen-linked carbohydrates. Different mechanisms were found to operate in hepatocyte attachment to collagen and to AC; the latter is most likely mediated by the hepatocyte surface receptor involved in recognition and uptake of asialoglycoproteins. The role of CIG in hepatocyte attachment to collagen was investigated. Data are presented suggesting that this glycoprotein, which mediates the adhesion of fibroblasts to collagen, is not required for hepatocyte attachment to collagen.
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U2 - 10.1016/0014-4827(78)90439-1
DO - 10.1016/0014-4827(78)90439-1
M3 - Article
C2 - 720402
AN - SCOPUS:0018082311
SN - 0014-4827
VL - 117
SP - 165
EP - 177
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -