Adhesion of glycosaminoglycan-deficient Chinese hamster ovary cell mutants to fibronectin substrata

R. G. LeBaron, J. D. Esko, A. Woods, S. Johansson, M. Hook

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

We have examined the role of cell surface glycosaminoglycans in fibronectin-mediated cell adhesion by analyzing the adhesive properties of Chinese hamster ovary cell mutants deficient in glycosaminoglycans. The results of our study suggest that the absence of glycosaminoglycans does not affect the initial attachment and subsequent spreading of these cells on substrata composed of intact fibronectin or a fibronectin fragment containing the primary cell-binding domain. However, in contrast to wild-type cells, the glycosaminoglycan-deficient cells did not attach to substrata composed of a heparin-binding fibronectin fragment. Furthermore, the wild-type but not the glycosaminoglycan-deficient cells formed F-actin-containing stress fibers and focal adhesions on substrata composed of intact fibronectin. We propose, therefore, that cell surface proteoglycan(s) participate in the transmembrane linking of intracellular cytoskeletal components to extracellular matrix components which occurs in focal adhesions.

Original languageEnglish (US)
Pages (from-to)945-952
Number of pages8
JournalJournal of Cell Biology
Volume106
Issue number3
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Cell Biology

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